PMID- 35081303
OWN - NLM
STAT- MEDLINE
DCOM- 20220808
LR  - 20240215
IS  - 2233-6087 (Electronic)
IS  - 2233-6079 (Print)
IS  - 2233-6079 (Linking)
VI  - 46
IP  - 4
DP  - 2022 Jul
TI  - Effect of Low-Dose Persistent Organic Pollutants on Mitochondrial Function: Human 
      and in Vitro Evidence.
PG  - 592-604
LID - 10.4093/dmj.2021.0132 [doi]
AB  - BACKGROUND: Chronic exposure to low-dose persistent organic pollutants (POPs) can 
      induce mitochondrial dysfunction. This study evaluated the association between 
      serum POP concentrations and oxygen consumption rate (OCR) as a marker of 
      mitochondrial function in humans and in vitro cells. METHODS: Serum 
      concentrations of organochlorine pesticides (OCPs) and polychlorinated biphenyls 
      (PCBs) were measured in 323 adults. The OCRs of platelets and peripheral blood 
      mononuclear cells (PBMCs) were assessed in 20 mL of fresh blood using a Seahorse 
      XF analyzer. Additionally, the in vitro effects of Arochlor-1254, 
      beta-hexachlorocyclohexane, and p,p -dichlorodiphenyltrichloroethane at 
      concentrations of 0.1 pM to 100 nM were evaluated in human platelets, human 
      PBMCs, and Jurkat T-cells. RESULTS: The association between serum POP 
      concentrations and OCR differed depending on the cell type. As serum OCP 
      concentrations increased, basal platelet OCR levels decreased significantly; 
      according to the OCP quintiles of summary measure, they were 8.6, 9.6, 8.2, 8.0, 
      and 7.1 pmol/min/mug (P trend=0.005). Notably, the basal PBMC OCR levels decreased 
      remarkably as the serum PCB concentration increased. PBMC OCR levels were 46.5, 
      34.3, 29.1, 16.5, and 13.1 pmol/min/mug according to the PCB quintiles of summary 
      measure (P trend <0.001), and this inverse association was consistently observed 
      in all subgroups stratified by age, sex, obesity, type 2 diabetes mellitus, and 
      hypertension, respectively. In vitro experimental studies have also demonstrated 
      that chronic exposure to low-dose POPs could decrease OCR levels. CONCLUSION: The 
      findings from human and in vitro studies suggest that chronic exposure to 
      low-dose POPs can induce mitochondrial dysfunction by impairing oxidative 
      phosphorylation.
FAU - Kim, Se-A
AU  - Kim SA
AD  - Department of Biomedical Science, Graduate School, Kyungpook National University, 
      Daegu, Korea.
FAU - Lee, Hoyul
AU  - Lee H
AD  - Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu, 
      Korea.
AD  - Leading-Edge Research Center for Drug Discovery and Development for Diabetes and 
      Metabolic Disease, Kyungpook National University Hospital, Daegu, Korea.
FAU - Park, Sung-Mi
AU  - Park SM
AD  - Leading-Edge Research Center for Drug Discovery and Development for Diabetes and 
      Metabolic Disease, Kyungpook National University Hospital, Daegu, Korea.
FAU - Kim, Mi-Jin
AU  - Kim MJ
AD  - Research Institute of Aging and Metabolism, Kyungpook National University, Daegu, 
      Korea.
FAU - Lee, Yu-Mi
AU  - Lee YM
AD  - Department of Preventive Medicine, School of Medicine, Kyungpook National 
      University, Daegu, Korea.
FAU - Yoon, Young-Ran
AU  - Yoon YR
AD  - BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, 
      Kyungpook National University, Daegu, Korea.
AD  - Department of Biomedical Science, Kyungpook National University Hospital, School 
      of Medicine, Kyungpook National University, Daegu, Korea.
FAU - Lee, Hyun-Kyung
AU  - Lee HK
AD  - Department of Marine Science and Convergence Engineering, College of Science and 
      Convergence Technology, Hanyang University, Ansan, Korea.
FAU - Moon, Hyo-Bang
AU  - Moon HB
AD  - Department of Marine Science and Convergence Engineering, College of Science and 
      Convergence Technology, Hanyang University, Ansan, Korea.
FAU - Lee, In-Kyu
AU  - Lee IK
AD  - Leading-Edge Research Center for Drug Discovery and Development for Diabetes and 
      Metabolic Disease, Kyungpook National University Hospital, Daegu, Korea.
AD  - Research Institute of Aging and Metabolism, Kyungpook National University, Daegu, 
      Korea.
AD  - Department of Internal Medicine, Kyungpook National University Hospital, School 
      of Medicine, Kyungpook National University, Daegu, Korea.
FAU - Lee, Duk-Hee
AU  - Lee DH
AD  - Department of Biomedical Science, Graduate School, Kyungpook National University, 
      Daegu, Korea.
AD  - Department of Preventive Medicine, School of Medicine, Kyungpook National 
      University, Daegu, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220126
PL  - Korea (South)
TA  - Diabetes Metab J
JT  - Diabetes & metabolism journal
JID - 101556588
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Adult
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Environmental Exposure/adverse effects
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Mitochondria/metabolism
MH  - Persistent Organic Pollutants
MH  - *Polychlorinated Biphenyls/metabolism/toxicity
PMC - PMC9353568
OTO - NOTNLM
OT  - Mitochondria
OT  - Oxygen consumption
OT  - Persistent organic pollutants
OT  - Pesticides
OT  - Polychlorinated biphenyls
COIS- CONFLICTS OF INTEREST No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2022/01/27 06:00
MHDA- 2022/08/09 06:00
PMCR- 2022/07/01
CRDT- 2022/01/26 18:28
PHST- 2021/06/24 00:00 [received]
PHST- 2021/08/20 00:00 [accepted]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/08/09 06:00 [medline]
PHST- 2022/01/26 18:28 [entrez]
PHST- 2022/07/01 00:00 [pmc-release]
AID - dmj.2021.0132 [pii]
AID - dmj-2021-0132 [pii]
AID - 10.4093/dmj.2021.0132 [doi]
PST - ppublish
SO  - Diabetes Metab J. 2022 Jul;46(4):592-604. doi: 10.4093/dmj.2021.0132. Epub 2022 
      Jan 26.