PMID- 35081486
OWN - NLM
STAT- MEDLINE
DCOM- 20220222
LR  - 20220222
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 202
DP  - 2022 Apr 15
TI  - Multielectrode biosensor chip for spatial resolution screening of 3D cell models 
      based on microcavity arrays.
PG  - 114010
LID - S0956-5663(22)00050-1 [pii]
LID - 10.1016/j.bios.2022.114010 [doi]
AB  - Three-dimensional cell models represent the native in vivo situation more closely 
      than two-dimensional cultures and are therefore preferred today for in vitro 
      studies. In this context, there is a great demand for fast, non-invasive, 
      real-time, and label-free methods that are capable for detailed analyses of 
      three-dimensional cultures. To characterize heterogeneous cultures or to detect 
      localized drug effects, a measurement method such as impedance spectroscopy in 
      combination with microcavity arrays (MCAs) is desirable, which additionally 
      offers spatial resolution. To overcome these limitations of the previously 
      described MCA based on opaque silicon substrates and a square shape with four 
      measurement electrodes imposed by the crystal structure, we used the selective 
      laser etching (SLE) method to fabricate microcavities in fused silica and 
      borosilicate glass without geometric constraints. We successfully developed MCAs 
      with variable base including up to eight measurement electrodes in one cavity, 
      which allows the increase in the number of electrode combinations to improve 
      spatial resolution. In addition, we integrated a central cone electrode at the 
      cavity bottom to extend the spatial resolution on the z-axis. To demonstrate the 
      capability of the MCAs, we used MDA-HB-231 spheroids with an enclosed glass 
      sphere to show that the heterogeneity of the model is evident in the relative 
      impedance spectra. Analyses on various cell spheroids highlight the broad 
      applicability of glass MCAs. In conclusion, our SLE-fabricated MCA clearly 
      improve bioelectronic analyses of cellular changes in heterogeneous 3D models. 
      Thus, bioelectronic analysis of electrophysiologically active cells and tumor 
      biopsy samples could significantly benefit from our development.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Zitzmann, Franziska D
AU  - Zitzmann FD
AD  - Center for Biotechnology and Biomedicine, Molecular Biological-biochemical 
      Processing Technology, Leipzig University, Deutscher Platz 5, D-04103, Leipzig, 
      Germany.
FAU - Schmidt, Sabine
AU  - Schmidt S
AD  - Center for Biotechnology and Biomedicine, Molecular Biological-biochemical 
      Processing Technology, Leipzig University, Deutscher Platz 5, D-04103, Leipzig, 
      Germany.
FAU - Naumann, Max
AU  - Naumann M
AD  - Center for Biotechnology and Biomedicine, Molecular Biological-biochemical 
      Processing Technology, Leipzig University, Deutscher Platz 5, D-04103, Leipzig, 
      Germany.
FAU - Belder, Detlev
AU  - Belder D
AD  - Institute of Analytical Chemistry, Leipzig University, Linnestrasse 3, 04103, 
      Leipzig, Germany.
FAU - Jahnke, Heinz-Georg
AU  - Jahnke HG
AD  - Center for Biotechnology and Biomedicine, Molecular Biological-biochemical 
      Processing Technology, Leipzig University, Deutscher Platz 5, D-04103, Leipzig, 
      Germany. Electronic address: heinz-georg.jahnke@bbz.uni-leipzig.de.
FAU - Robitzki, Andrea A
AU  - Robitzki AA
AD  - Center for Biotechnology and Biomedicine, Molecular Biological-biochemical 
      Processing Technology, Leipzig University, Deutscher Platz 5, D-04103, Leipzig, 
      Germany; Karlsruher Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 
      76344, Eggenstein-Leopoldshafen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20220119
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - *Biosensing Techniques/methods
MH  - Dielectric Spectroscopy
MH  - Electric Impedance
MH  - Electrodes
MH  - Spheroids, Cellular
OTO - NOTNLM
OT  - 3D cell culture
OT  - Electrochemical impedance spectroscopy
OT  - Field potential recording
OT  - MDA-MB-231
OT  - Microcavity array technology
OT  - Selective laser etching
EDAT- 2022/01/27 06:00
MHDA- 2022/02/23 06:00
CRDT- 2022/01/26 20:07
PHST- 2021/10/25 00:00 [received]
PHST- 2021/12/24 00:00 [revised]
PHST- 2022/01/14 00:00 [accepted]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/02/23 06:00 [medline]
PHST- 2022/01/26 20:07 [entrez]
AID - S0956-5663(22)00050-1 [pii]
AID - 10.1016/j.bios.2022.114010 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2022 Apr 15;202:114010. doi: 10.1016/j.bios.2022.114010. 
      Epub 2022 Jan 19.