PMID- 35081536
OWN - NLM
STAT- MEDLINE
DCOM- 20220517
LR  - 20220531
IS  - 1423-0143 (Electronic)
IS  - 1420-4096 (Linking)
VI  - 47
IP  - 5
DP  - 2022
TI  - Mechanism of miRNA-141-3p in Calcium Oxalate-Induced Renal Tubular Epithelial 
      Cell Injury via NLRP3-Mediated Pyroptosis.
PG  - 300-308
LID - 10.1159/000521795 [doi]
AB  - BACKGROUND/AIMS: Renal calculi represent a prevalent disorder associated with 
      mineral deposition in renal calyces and the pelvis. Aberrant microRNA (miRNA) 
      expression is implicated in renal injury. This study investigated the mechanism 
      of miR-141-3p in calcium oxalate (CaOx) crystal-induced renal tubular epithelial 
      cell (RTEC) injury. METHODS: Human RTECs HK-2 cells were treated with CaOx 
      crystals to induce RTEC injury. Cell viability was evaluated using Cell Counting 
      Kit-8 assay, and apoptosis was measured using flow cytometry. The contents of 
      lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), 
      interleukin (IL)-1beta, and IL-18 were measured using enzyme-linked immunosorbent 
      assay kits. The expressions of NLRP3, cleaved caspase-1, and GSDMD-N were 
      detected using Western blot. miR-141-3p and NLRP3 expressions were determined 
      using reverse transcription quantitative polymerase chain reaction. The binding 
      of miR-141-3p and NLRP3 was validated using a dual-luciferase assay. The role of 
      NLRP3 in the protection of miR-141-3p on RTEC injury was verified using 
      functional rescue experiments. RESULTS: CaOx crystals induced RTEC injury, 
      manifested as attenuated cell viability, enhanced apoptosis, elevated 
      intracellular LDH and MDA levels, and decreased SOD level. Pyroptosis of RTECs 
      was enhanced by CaOx crystal induction, evidenced by elevated expressions of 
      cleaved caspase-1, GSDMD-N, IL-1beta, and IL-18. miR-141-3p expression was reduced 
      in CaOx crystal-induced RTECs. miR-141-3p overexpression alleviated CaOx 
      crystal-induced RTEC injury and suppressed pyroptosis of RTECs. miR-141-3p bound 
      to NLRP3 and thereby repressed NLRP3 expression. NLRP3 overexpression reversed 
      the protective effect of miR-141-3p overexpression on RTECs. CONCLUSION: 
      miR-141-3p repressed NLRP3-mediated pyroptosis by suppressing NLRP3 expression, 
      thus protecting CaOx crystal-induced RTEC injury.
CI  - (c) 2022 The Author(s). Published by S. Karger AG, Basel.
FAU - Gan, Xiu-Guo
AU  - Gan XG
AD  - Department of Urology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, China.
FAU - Wang, Zhi-Hao
AU  - Wang ZH
AD  - Department of Urology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, China.
FAU - Xu, Hai-Tao
AU  - Xu HT
AD  - Department of Urology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20220126
PL  - Switzerland
TA  - Kidney Blood Press Res
JT  - Kidney & blood pressure research
JID - 9610505
RN  - 0 (Interleukin-18)
RN  - 0 (MIRN141 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - *Calcium Oxalate/metabolism
MH  - Caspase 1/metabolism
MH  - Epithelial Cells/metabolism/pathology
MH  - Humans
MH  - Interleukin-18/metabolism
MH  - *Kidney Calculi/metabolism/pathology
MH  - Male
MH  - *MicroRNAs/metabolism
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Pyroptosis
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Calcium oxalate
OT  - Pyroptosis
OT  - Renal calculi
OT  - Renal tubular epithelial cells
OT  - miR-141-3p
EDAT- 2022/01/27 06:00
MHDA- 2022/05/18 06:00
CRDT- 2022/01/26 20:07
PHST- 2021/10/13 00:00 [received]
PHST- 2022/01/04 00:00 [accepted]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/05/18 06:00 [medline]
PHST- 2022/01/26 20:07 [entrez]
AID - 000521795 [pii]
AID - 10.1159/000521795 [doi]
PST - ppublish
SO  - Kidney Blood Press Res. 2022;47(5):300-308. doi: 10.1159/000521795. Epub 2022 Jan 
      26.