PMID- 35082082
OWN - NLM
STAT- MEDLINE
DCOM- 20220128
LR  - 20220128
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 103
DP  - 2022 May
TI  - Spatial organisation of Listeria monocytogenes and Escherichia coli O157:H7 
      cultivated in gel matrices.
PG  - 103965
LID - S0740-0020(21)00231-8 [pii]
LID - 10.1016/j.fm.2021.103965 [doi]
AB  - The spatial organisation of bacterial pathogens in food matrices remains poorly 
      understood, but is important in improving risk assessment and preventing 
      infection of consumers by contaminated foodstuff. By combining confocal laser 
      scanning microscopy with genetic fluorescent labelling of Listeria monocytogenes 
      and Escherichia coli O157:H7, it was possible to investigate the spatial patterns 
      of colonisation of both foodborne pathogens in gel matrices, alone or in 
      combination, in various environmental conditions. Increasing low melting point 
      agarose (LMPA) concentrations triggers the transition between a motile 
      single-cell lifestyle to a sessile population spatially organised as 
      microcolonies. The size, number and morphology of microcolonies were highly 
      affected by supplementations in NaCl or lactic acid, two compounds frequently 
      used in food products. Strikingly, single-cell motility was partially restored at 
      higher LMPA concentration in the presence of lactic acid for Escherichia coli 
      O157:H7 and in the presence of NaCl for Listeria monocytogenes. Co-culture of 
      both species in the hydrogel affected pathogen colonisation features; Listeria 
      monocytogenes was better able to colonise gel matrices containing lactic acid in 
      the presence of Escherichia coli O157:H7. Altogether, this investigation provides 
      insights into the spatial distribution and structural dynamics of bacterial 
      pathogens in gel matrices. Potential impacts on food safety are discussed.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Saint Martin, Cedric
AU  - Saint Martin C
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France; Universite Clermont Auvergne, INRAE, UMR454 MEDIS, 63000, 
      Clermont-Ferrand, France.
FAU - Darsonval, Maud
AU  - Darsonval M
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France.
FAU - Gregoire, Marina
AU  - Gregoire M
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France.
FAU - Caccia, Nelly
AU  - Caccia N
AD  - Universite Clermont Auvergne, INRAE, UMR454 MEDIS, 63000, Clermont-Ferrand, 
      France.
FAU - Midoux, Lucas
AU  - Midoux L
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France.
FAU - Berland, Sophie
AU  - Berland S
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, SayFood, 91300, Massy, France.
FAU - Leroy, Sabine
AU  - Leroy S
AD  - Universite Clermont Auvergne, INRAE, UMR454 MEDIS, 63000, Clermont-Ferrand, 
      France.
FAU - Dubois-Brissonnet, Florence
AU  - Dubois-Brissonnet F
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France.
FAU - Desvaux, Mickael
AU  - Desvaux M
AD  - Universite Clermont Auvergne, INRAE, UMR454 MEDIS, 63000, Clermont-Ferrand, 
      France. Electronic address: mickael.desvaux@inrae.fr.
FAU - Briandet, Romain
AU  - Briandet R
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, MICALIS Institute, 78350, 
      Jouy-en-Josas, France. Electronic address: romain.briandet@inrae.fr.
LA  - eng
PT  - Journal Article
DEP - 20211208
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
SB  - IM
MH  - Colony Count, Microbial
MH  - *Escherichia coli O157/genetics
MH  - Food Microbiology
MH  - *Listeria monocytogenes/genetics
OTO - NOTNLM
OT  - Bacterial motility
OT  - Confocal laser scanning microscopy
OT  - Escherichia coli O157:H7
OT  - Food matrix
OT  - Hydrogel
OT  - Listeria monocytogenes
OT  - Spatial organisation
EDAT- 2022/01/28 06:00
MHDA- 2022/01/29 06:00
CRDT- 2022/01/27 05:36
PHST- 2021/08/11 00:00 [received]
PHST- 2021/12/03 00:00 [revised]
PHST- 2021/12/06 00:00 [accepted]
PHST- 2022/01/27 05:36 [entrez]
PHST- 2022/01/28 06:00 [pubmed]
PHST- 2022/01/29 06:00 [medline]
AID - S0740-0020(21)00231-8 [pii]
AID - 10.1016/j.fm.2021.103965 [doi]
PST - ppublish
SO  - Food Microbiol. 2022 May;103:103965. doi: 10.1016/j.fm.2021.103965. Epub 2021 Dec 
      8.