PMID- 35084728
OWN - NLM
STAT- Publisher
LR  - 20240220
IS  - 2543-6031 (Electronic)
IS  - 2451-4934 (Linking)
DP  - 2022 Jan 27
TI  - The effect of vitamin D on GATA3 gene expression in peripheral blood mononuclear 
      cells in allergic asthma.
LID - 10.5603/ARM.a2022.0004 [doi]
AB  - INTRODUCTION: Asthma is becoming a major health problem in many countries. Immune 
      responses in allergic asthma, as the most prevalent asthmatic phenotype, are 
      mediated mostly by a subtype of T lymphocytes referred to as the effector lineage 
      of Type 2 Th cells (Th2). The development of Th2 cells is mainly governed by a 
      zinc finger transcription factor, i.e., GATA-binding protein 3 (GATA3). Allergic 
      asthma is a complex disease, and vitamin D deficiency has been named as a 
      non-genetic risk factor for its development. Vitamin D, a steroid hormone 
      belonging to the family of nuclear receptors, has shown significant 
      immunosuppressive effects in previous studies. In this study, given its 
      immunomodulatory properties, we aimed to investigate the effects of different 
      concentrations of vitamin D on GATA3 gene expression in peripheral blood 
      mononuclear cells (PBMCs), including Th2 cells, and compare GATA3 expression 
      levels between PBMCs taken from allergic asthmatic patients and healthy controls. 
      RESULTS: The total sample size was 40 and the quantitative real-time PCR (qPCR) 
      procedure was applied to assess the mRNA expression levels of GATA3 in different 
      groups. Collectively, our results demonstrated that the expression of GATA3 in 
      PBMCs taken from patients with allergic asthma is lower than in that from healthy 
      controls. In addition, in the control group, cells co-cultured with vitamin D had 
      a significantly increased GATA3 expression. However, in the patient group, such 
      an increase was only observed in cells treated with 10(-)(7)M-vitamin D. By contrast, 
      incubation with vitamin D at the concentration of 10-6 M slightly decreased the 
      expression of GATA3 among patients. CONCLUSION: In summary, it is likely that 
      vitamin D should regulate GATA3 gene expression in the PBMCs in a dose-dependent 
      manner. The impacts of this steroid hormone can also differ between the status of 
      health and allergic asthma in either extent or direction.
FAU - Erfanian, Saiedeh
AU  - Erfanian S
AD  - Department of Advanced Medical Sciences and Technologies, Research Center for 
      Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
FAU - Jafaripour, Simin
AU  - Jafaripour S
AD  - Department of Medical Genetics, School of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Jokar, Mohammad Hassan
AU  - Jokar MH
AD  - Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan 
      University of Medical Sciences, Gorgan, Iran.
FAU - Sedighi, Sima
AU  - Sedighi S
AD  - Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan 
      University of Medical Sciences, Gorgan, Iran.
FAU - Sotoodeh Jahromi, Abdolreza
AU  - Sotoodeh Jahromi A
AD  - Department of Advanced Medical Sciences and Technologies, Research Center for 
      Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
FAU - Razavi, Faezeh
AU  - Razavi F
AD  - Department of Advanced Medical Sciences and Technologies, Research Center for 
      Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
FAU - Farhang Zargar, Mohsen
AU  - Farhang Zargar M
AD  - Department of Advanced Medical Sciences and Technologies, Research Center for 
      Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
FAU - Moradzadeh, Maliheh
AU  - Moradzadeh M
AD  - Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan 
      University of Medical Sciences, Gorgan, Iran. moradzadeh63@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20220127
PL  - Switzerland
TA  - Adv Respir Med
JT  - Advances in respiratory medicine
JID - 101697329
SB  - IM
OTO - NOTNLM
OT  - GATA3
OT  - Th2
OT  - allergic asthma
OT  - immune response
OT  - vitamin D
EDAT- 2022/01/28 06:00
MHDA- 2022/01/28 06:00
CRDT- 2022/01/27 12:18
PHST- 2021/03/28 00:00 [received]
PHST- 2021/12/14 00:00 [accepted]
PHST- 2021/12/06 00:00 [revised]
PHST- 2022/01/27 12:18 [entrez]
PHST- 2022/01/28 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
AID - ARM.a2022.0004 [pii]
AID - 10.5603/ARM.a2022.0004 [doi]
PST - aheadofprint
SO  - Adv Respir Med. 2022 Jan 27. doi: 10.5603/ARM.a2022.0004.