PMID- 35086476 OWN - NLM STAT- MEDLINE DCOM- 20220131 LR - 20220204 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 23 IP - 1 DP - 2022 Jan 27 TI - Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types. PG - 82 LID - 10.1186/s12864-022-08323-z [doi] LID - 82 AB - BACKGROUND: The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specific gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells. RESULTS: Endometrial LE, GE, and ST cells show specific transcriptomic profiles. Most of the differentially expressed genes (DEGs) in response to progesterone are cell type-specific (96%). Genes involved in cell cycle and nuclear division are under-expressed in the presence of progesterone in GE, highlighting the anti-proliferative action of progesterone in epithelial cells. Elevated progesterone concentrations are also associated with the under-expression of estrogen receptor 1 (ESR1) in GE and oxytocin receptor (OXTR) in GE and ST cells. In ST cells, transcription factors such as SOX17 and FOXA2, known to regulate uterine epithelial-stromal cross-talk conveying to endometrial receptivity, are over-expressed under progesterone influence. CONCLUSIONS: The results from this study show that progesterone regulates endometrial function in a cell type-specific way, which is independent of the expression of its main receptor PGR. These novel insights into uterine physiology present the cell compartment as the physiological unit rather than the whole tissue. CI - (c) 2022. The Author(s). FAU - Pereira, Goncalo AU - Pereira G AD - CIISA-Centro de Investigacao Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Tecnica, 1300-477, Lisbon, Portugal. FAU - Guo, Yongzhi AU - Guo Y AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences, SLU, PO Box 7054, 750 07, Uppsala, Sweden. FAU - Silva, Elisabete AU - Silva E AD - CIISA-Centro de Investigacao Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Tecnica, 1300-477, Lisbon, Portugal. FAU - Bevilacqua, Claudia AU - Bevilacqua C AD - Universite Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. FAU - Charpigny, Gilles AU - Charpigny G AD - Universite Paris-Saclay, INRAE, ENVA, BREED, 78350, Jouy-en-Josas, France. FAU - Lopes-da-Costa, Luis AU - Lopes-da-Costa L AD - CIISA-Centro de Investigacao Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Tecnica, 1300-477, Lisbon, Portugal. lcosta@fmv.ulisboa.pt. FAU - Humblot, Patrice AU - Humblot P AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences, SLU, PO Box 7054, 750 07, Uppsala, Sweden. LA - eng PT - Journal Article DEP - 20220127 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 RN - 0 (Receptors, Progesterone) RN - 4G7DS2Q64Y (Progesterone) SB - IM MH - Animals MH - Cattle MH - Endometrium MH - Female MH - *Progesterone/pharmacology MH - Receptors, Progesterone/genetics MH - Stromal Cells MH - *Transcriptome MH - Uterus PMC - PMC8793221 OTO - NOTNLM OT - Endometrium OT - LCM OT - Progesterone OT - RNA-seq OT - Transcriptome COIS- The authors declare that they have no competing interests. EDAT- 2022/01/29 06:00 MHDA- 2022/02/01 06:00 PMCR- 2022/01/27 CRDT- 2022/01/28 05:33 PHST- 2021/09/06 00:00 [received] PHST- 2022/01/20 00:00 [accepted] PHST- 2022/01/28 05:33 [entrez] PHST- 2022/01/29 06:00 [pubmed] PHST- 2022/02/01 06:00 [medline] PHST- 2022/01/27 00:00 [pmc-release] AID - 10.1186/s12864-022-08323-z [pii] AID - 8323 [pii] AID - 10.1186/s12864-022-08323-z [doi] PST - epublish SO - BMC Genomics. 2022 Jan 27;23(1):82. doi: 10.1186/s12864-022-08323-z.