PMID- 35087736
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240405
IS  - 2234-8646 (Print)
IS  - 2234-8840 (Electronic)
IS  - 2234-8840 (Linking)
VI  - 25
IP  - 1
DP  - 2022 Jan
TI  - Zinc Deficiency Elevates Fecal Protein, But Not Electrolyte and Short-Chain Fatty 
      Acid, Levels in Enterotoxigenic Escherichia coli-Induced Diarrhea in Rats.
PG  - 79-86
LID - 10.5223/pghn.2022.25.1.79 [doi]
AB  - PURPOSE: To determine the effect of zinc deficiency on fecal protein, 
      electrolyte, and short-chain fatty acid levels in both heat-stable (ST) and 
      heat-labile (LT) enterotoxigenic Escherichia coli (ETEC)-induced diarrhea in 
      rats. METHODS: Albino rats, weighing 100 to 150 g, were divided into 2 groups, 
      with 15 animals each: non-zinc and zinc-deficient. These two groups were 
      sub-divided into three sub-groups with five rats each: control (saline); LT-ETEC; 
      and ST-ETEC. Sodium phytate (30 mmol/L) was added to the animals' water to induce 
      zinc deficiency, while diarrhea was induced using 5x10(9) ETEC cells/mL. Fecal 
      protein levels were estimated using the Bradford method, while sodium and 
      potassium levels were determined using atomic absorption spectrophotometry. 
      Short-chain fatty acids were measured using gas chromatography-mass spectrometry. 
      RESULTS: Among the non-zinc and zinc-deficient groups, there were significant 
      increases (p=0.04), (p=0.03) in fecal protein concentrations (mg/mL) in the 
      LT-ETEC- (4.50+/-0.33), (6.50+/-0.26) and ST-ETEC- (3.85+/-0.19), (5.98+/-0.32) induced 
      groups compared to the control groups (2.60+/-0.52), (3.50+/-0.11) respectively. 
      Fecal sodium and potassium levels (mg/L) were significantly (p=0.029) increased 
      in non-zinc-deficient rats induced with LT-ETEC (9.35+/-0.95, 1.05+/-0.48), and 
      ST-ETEC (9.96+/-1.02, 1.21+/-0.45) compared with the control group (8.07+/-0.44, 
      0.47+/-0.17) but the increase were not statistically significant (p=0.059) in the 
      zinc deficient rat groups. Fecal acetate and propionate levels (mg/g) 
      significantly (p=0.032) increased when induced with LT-ETEC and ST-ETEC in 
      non-zinc and zinc-deficient groups compared with the control groups. CONCLUSION: 
      Zinc deficiency among rats with ETEC-induced diarrhea elevated fecal protein loss 
      but may not have an effect on fecal sodium, potassium and short-chain fatty acid 
      levels.
CI  - Copyright (c) 2022 by The Korean Society of Pediatric Gastroenterology, Hepatology 
      and Nutrition.
FAU - David, Ebuka E
AU  - David EE
AUID- ORCID: 0000-0001-6903-6392
AD  - Department of Biochemistry, Alex Ekwueme Federal University Ndufu-Alike Ikwo, 
      Ebonyi State, Nigeria.
FAU - Yameen, Muhammad A
AU  - Yameen MA
AUID- ORCID: 0000-0003-3596-454X
AD  - Department of Pharmacy, COMSATS University, Abbottabad Campus, Pakistan.
FAU - Igwenyi, Ikechuku O
AU  - Igwenyi IO
AUID- ORCID: 0000-0002-2056-751X
AD  - Department of Biochemistry, Ebonyi State University, Ebonyi State, Nigeria.
FAU - David, Chidinma N
AU  - David CN
AUID- ORCID: 0000-0002-2550-4614
AD  - Department of Microbiology, Ebonyi State University, Ebonyi State, Nigeria.
FAU - Nwobodo, Valentine
AU  - Nwobodo V
AUID- ORCID: 0000-0002-4899-8258
AD  - Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra 
      State, Nigeria.
FAU - Ismail, Akindele K
AU  - Ismail AK
AUID- ORCID: 0000-0003-2210-1796
AD  - Department of Microbiology, Alex Ekwueme Federal University Ndufu-Alike Ikwo, 
      Ebonyi State, Nigeria.
LA  - eng
PT  - Journal Article
DEP - 20220107
PL  - Korea (South)
TA  - Pediatr Gastroenterol Hepatol Nutr
JT  - Pediatric gastroenterology, hepatology & nutrition
JID - 101590471
PMC - PMC8762603
OTO - NOTNLM
OT  - Diarrhea
OT  - Enterotoxigenic Escherichia coli
OT  - Fatty acids, Volatile
OT  - Zinc
COIS- Conflict of Interest: The authors have no financial conflicts of interest.
EDAT- 2022/01/29 06:00
MHDA- 2022/01/29 06:01
PMCR- 2022/01/01
CRDT- 2022/01/28 05:54
PHST- 2020/10/12 00:00 [received]
PHST- 2021/10/09 00:00 [revised]
PHST- 2021/12/17 00:00 [accepted]
PHST- 2022/01/28 05:54 [entrez]
PHST- 2022/01/29 06:00 [pubmed]
PHST- 2022/01/29 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.5223/pghn.2022.25.1.79 [doi]
PST - ppublish
SO  - Pediatr Gastroenterol Hepatol Nutr. 2022 Jan;25(1):79-86. doi: 
      10.5223/pghn.2022.25.1.79. Epub 2022 Jan 7.