PMID- 35087752
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220430
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - Identification of Novel Prognostic Markers Associated With Laryngeal Squamous 
      Cell Carcinoma Using Comprehensive Analysis.
PG  - 779153
LID - 10.3389/fonc.2021.779153 [doi]
LID - 779153
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is a leading malignant 
      cancer of the head and neck. Patients with LSCC, in which the cancer has 
      infiltrated and metastasized, have a poor prognosis. Therefore, there is an 
      urgent need to identify more potential targets for drugs and biomarkers for early 
      diagnosis. METHODS: RNA sequence data from LSCC and patients' clinical traits 
      were obtained from the Gene Expression Omnibus (GEO) (GSE142083) and The Cancer 
      Genome Atlas (TCGA) database. Differentially expressed gene (DEG) analysis and 
      weighted gene co-expression network analysis (WGCNA) were performed to identify 
      hub genes. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      analysis, prognostic value analysis, receiver operating characteristic (ROC) 
      curve analysis, gene mutation analysis, tumor-infiltrating immune cell abundance 
      profile estimation, gene set variation analysis (GSVA), and gene set enrichment 
      analysis (GSEA) were performed. Single-gene RNA sequencing data were obtained 
      from the GSE150321 dataset. Cell proliferation and viability were confirmed by 
      the CCK-8 assay and real-time PCR. RESULTS: A total of 701 DEGs, including 329 
      upregulated and 372 downregulated genes, were screened in the GSE142083 dataset. 
      Using WGCNA, three modules were identified to be closely related to LSCC. After 
      intersecting the DEGs and performing univariate and multivariate Cox analyses, a 
      novel prognostic model based on three genes (SLC35C1, HOXB7, and TEDC2) for LSCC 
      was established. Interfering TEDC2 expression inhibited tumor cell proliferation 
      and migration. CONCLUSIONS: Our results show that SLC35C1, HOXB7, and TEDC2 have 
      the potential to become new therapeutic targets and prognostic biomarkers for 
      LSCC.
CI  - Copyright (c) 2022 Huang, He, Dong, Huang, Chen, Peng and Huang.
FAU - Huang, Chao
AU  - Huang C
AD  - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital 
      Central South University, Changsha, China.
FAU - He, Jun
AU  - He J
AD  - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital 
      Central South University, Changsha, China.
FAU - Dong, Yi
AU  - Dong Y
AD  - Department of Nephrology, Xiangya Hospital Central South University, Changsha, 
      China.
AD  - Department of Cell Biology, School of Life Sciences, Central South University, 
      Changsha, China.
AD  - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, 
      China.
FAU - Huang, Li
AU  - Huang L
AD  - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital 
      Central South University, Changsha, China.
FAU - Chen, Yichao
AU  - Chen Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital 
      Central South University, Changsha, China.
FAU - Peng, Anquan
AU  - Peng A
AD  - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital 
      Central South University, Changsha, China.
FAU - Huang, Hao
AU  - Huang H
AD  - Department of Nephrology, Xiangya Hospital Central South University, Changsha, 
      China.
AD  - Department of Cell Biology, School of Life Sciences, Central South University, 
      Changsha, China.
AD  - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, 
      China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital 
      Central South University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20220111
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8787159
OTO - NOTNLM
OT  - TEDC2
OT  - WGCNA
OT  - differentially expressed genes
OT  - gene modules
OT  - laryngeal squamous cell carcinoma
OT  - prognostic gene
OT  - single-cell analysis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/01/29 06:00
MHDA- 2022/01/29 06:01
PMCR- 2021/01/01
CRDT- 2022/01/28 05:54
PHST- 2021/09/20 00:00 [received]
PHST- 2021/12/13 00:00 [accepted]
PHST- 2022/01/28 05:54 [entrez]
PHST- 2022/01/29 06:00 [pubmed]
PHST- 2022/01/29 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.779153 [doi]
PST - epublish
SO  - Front Oncol. 2022 Jan 11;11:779153. doi: 10.3389/fonc.2021.779153. eCollection 
      2021.