PMID- 35088367
OWN - NLM
STAT- MEDLINE
DCOM- 20220328
LR  - 20220401
IS  - 1474-9092 (Electronic)
IS  - 1474-905X (Linking)
VI  - 21
IP  - 3
DP  - 2022 Mar
TI  - Supramolecular co-encapsulation of a photosensitizer and chemotherapeutic drug in 
      cucurbit[8]uril for potential chemophototherapy.
PG  - 349-359
LID - 10.1007/s43630-022-00174-7 [doi]
AB  - Supramolecular strategies as well as combinatorial approaches have been proposed 
      to improve cancer therapeutics. In this work, we investigated the encapsulation 
      of the photosensitizer acridine orange (AO) and the chemotherapeutic drug 
      oxaliplatin (OxPt) in cucurbit[8]uril (CB[8]), and tested their effect both 
      separate and combined on tumoral cells cultivated in vitro. Binding constants and 
      enthalpies of reaction for the AO@CB[8], (AO)(2)@CB[8] and OxPt@CB[8] complexes 
      were determined by isothermal titration calorimetry. In the case of AO, a 
      negative cooperativity for the binding of the second AO molecule was found, in 
      agreement with previous fluorescence titration data. We show herein that the 
      AO@CB[8] complex was effectively incorporated within the cells and showed 
      important phototoxicity, while the OxPt@CB[8] complex was cytotoxic only at long 
      incubation times (24 h). Pre-treatment of the cells with the OxPt@CB[8] complex 
      for 24 h inhibited any photodynamic action by the later treatment with the 
      AO@CB[8] complex. However, when both complexes were co-incubated for 90 min, the 
      combined cytotoxicity/phototoxicity was superior to any of the treatments 
      individually. A cooperative effect was identified that added up to an extra 30% 
      cytotoxicity/phototoxicity. The results point to an interesting system where a 
      photosensitizer and chemotherapeutic drug are co-encapsulated in a macrocycle to 
      develop chemophototherapy applications.
CI  - (c) 2022. The Author(s), under exclusive licence to European Photochemistry 
      Association, European Society for Photobiology.
FAU - Solis-Egana, Fresia
AU  - Solis-Egana F
AD  - Laboratorio de Quimica Supramolecular y Fotobiologia, Departamento de Quimica 
      Fisica, Escuela de Quimica, Facultad de Quimica y de Farmacia, Pontificia 
      Universidad Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile.
FAU - Lavin-Urqueta, Nicole
AU  - Lavin-Urqueta N
AD  - Laboratorio de Quimica Supramolecular y Fotobiologia, Departamento de Quimica 
      Fisica, Escuela de Quimica, Facultad de Quimica y de Farmacia, Pontificia 
      Universidad Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile.
FAU - Guerra Diaz, Daniel
AU  - Guerra Diaz D
AD  - Laboratorio de Quimica Supramolecular y Fotobiologia, Departamento de Quimica 
      Fisica, Escuela de Quimica, Facultad de Quimica y de Farmacia, Pontificia 
      Universidad Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile.
FAU - Marino-Ocampo, Nory
AU  - Marino-Ocampo N
AD  - Laboratorio de Quimica Supramolecular y Fotobiologia, Departamento de Quimica 
      Fisica, Escuela de Quimica, Facultad de Quimica y de Farmacia, Pontificia 
      Universidad Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile.
FAU - Faundez, Mario A
AU  - Faundez MA
AD  - Escuela de Farmacia, Facultad de Quimica y de Farmacia, Pontificia Universidad 
      Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile.
FAU - Fuentealba, Denis
AU  - Fuentealba D
AUID- ORCID: 0000-0003-4798-7204
AD  - Laboratorio de Quimica Supramolecular y Fotobiologia, Departamento de Quimica 
      Fisica, Escuela de Quimica, Facultad de Quimica y de Farmacia, Pontificia 
      Universidad Catolica de Chile, Vicuna Mackenna 4860, Macul, Santiago, Chile. 
      dlfuente@uc.cl.
LA  - eng
GR  - 1210583/FONDECYT/
GR  - EQM160042/FONDEQUIP/
GR  - EQM170120/FONDEQUIP/
GR  - PUENTE UC 014/2020/VRI/
PT  - Journal Article
DEP - 20220127
PL  - England
TA  - Photochem Photobiol Sci
JT  - Photochemical & photobiological sciences : Official journal of the European 
      Photochemistry Association and the European Society for Photobiology
JID - 101124451
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Bridged-Ring Compounds)
RN  - 0 (Heterocyclic Compounds, 2-Ring)
RN  - 0 (Imidazoles)
RN  - 0 (Imidazolidines)
RN  - 0 (Macrocyclic Compounds)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (cucurbit(8)uril)
SB  - IM
MH  - *Antineoplastic Agents/chemistry/pharmacology
MH  - Bridged-Ring Compounds/chemistry/pharmacology
MH  - Heterocyclic Compounds, 2-Ring
MH  - Imidazoles/chemistry/pharmacology
MH  - Imidazolidines
MH  - Macrocyclic Compounds
MH  - *Photosensitizing Agents/chemistry/pharmacology
OTO - NOTNLM
OT  - Acridine orange
OT  - Chemophototherapy
OT  - Cucurbit[8]uril
OT  - Oxaliplatin
OT  - Supramolecular
EDAT- 2022/01/29 06:00
MHDA- 2022/03/29 06:00
CRDT- 2022/01/28 05:57
PHST- 2021/11/16 00:00 [received]
PHST- 2022/01/14 00:00 [accepted]
PHST- 2022/01/29 06:00 [pubmed]
PHST- 2022/03/29 06:00 [medline]
PHST- 2022/01/28 05:57 [entrez]
AID - 10.1007/s43630-022-00174-7 [pii]
AID - 10.1007/s43630-022-00174-7 [doi]
PST - ppublish
SO  - Photochem Photobiol Sci. 2022 Mar;21(3):349-359. doi: 10.1007/s43630-022-00174-7. 
      Epub 2022 Jan 27.