PMID- 35091179
OWN - NLM
STAT- MEDLINE
DCOM- 20220328
LR  - 20220328
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 231
DP  - 2022 Mar
TI  - Diagnosis of giant cell-rich bone tumors on core needle biopsy: A practical 
      approach.
PG  - 153777
LID - S0344-0338(22)00020-6 [pii]
LID - 10.1016/j.prp.2022.153777 [doi]
AB  - BACKGROUND: The differential diagnosis of giant cell-rich bone tumors comprises a 
      broad spectrum of lesions with prominent reactive osteoclast-like and/or 
      neoplastic giant cells, with substantial differences in biologic behavior and 
      clinical management. Evaluation of giant cell-rich bone tumors on small biopsies 
      can be challenging especially in specimens with limited representative material. 
      An accurate diagnosis requires a high level of skill on the part of both 
      radiologist and pathologist as correlation with clinical and radiologic 
      characteristics is critical. The objective of the study was to assess the utility 
      of touch preparations (TP), immunohistochemistry (IHC) for mutation-specific 
      markers H3G34W and H3K36M, and fluorescence in situ hybridization (FISH) for USP6 
      rearrangements and MDM2 amplification in the diagnostic workup of core needle 
      biopsy specimens. METHODS: A total of 27 core needle biopsies with TPs from 
      patients with primary giant cell-rich bone tumors (16 giant cell tumors of bone 
      (GCTBs) (including 3 with lung metastasis), 3 chondroblastomas (CBs), 4 primary 
      aneurysmal bone cysts (ABCs), 2 non-ossifying fibromas (NOFs), 1 low grade 
      osteosarcoma (OS), and 1 conventional OS with tumor giant cells were analyzed 
      with IHC for H3G34W and H3K36M and in select cases FISH for USP6 rearrangements 
      and MDM2 amplification. RESULTS: In all cases the core biopsies were sufficient 
      for histologic examination and diagnostic workup. 16 of 16 GCTBs were positive 
      for H3G34W and negative for H3K36M, and 3 of 3 CBs were positive for H3K36M and 
      negative for H3G34W. All other cases were negative for H3G34W and H3K36M. 4 of 4 
      primary ABCs showed rearrangement of USP6 by FISH and the low grade OS showed 
      amplification of MDM2 by FISH. CONCLUSIONS: On-site adequacy assessment of TPs 
      proved to be an accurate, simple, and fast method for obtaining sufficient 
      material for complete diagnostic workup. The application of IHC for H3G34W and 
      H3K36M and FISH for detection of rearrangements of USP6 and amplification of MDM2 
      can improve the diagnostic accuracy in core needle biopsy specimens.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Jager, Lucy
AU  - Jager L
AD  - Department of Pathology, Northwestern University Feinberg School of Medicine, 
      Northwestern Memorial Hospital, 251 East Huron St, Feinberg 7-342A, Chicago, IL 
      60611, United States. Electronic address: lucy.jager@northwestern.edu.
FAU - Johnson, Daniel N
AU  - Johnson DN
AD  - Department of Pathology, Northwestern University Feinberg School of Medicine, 
      Northwestern Memorial Hospital, 251 East Huron St, Feinberg 7-342A, Chicago, IL 
      60611, United States.
FAU - Sukhanova, Madina
AU  - Sukhanova M
AD  - Department of Pathology, Northwestern University Feinberg School of Medicine, 
      Northwestern Memorial Hospital, 251 East Huron St, Feinberg 7-342A, Chicago, IL 
      60611, United States.
FAU - Streich, Lukas
AU  - Streich L
AD  - Department of Pathology, Northwestern University Feinberg School of Medicine, 
      Northwestern Memorial Hospital, 251 East Huron St, Feinberg 7-342A, Chicago, IL 
      60611, United States.
FAU - Chapa, Ajay R
AU  - Chapa AR
AD  - Musculoskeletal Imaging Section, Northwestern University Feinberg School of 
      Medicine, Northwestern Memorial Hospital, 676N Saint Clair St, Arkes Family 
      Pavilion, Chicago, IL 60611, United States.
FAU - Alexiev, Borislav A
AU  - Alexiev BA
AD  - Department of Pathology, Northwestern University Feinberg School of Medicine, 
      Northwestern Memorial Hospital, 251 East Huron St, Feinberg 7-342A, Chicago, IL 
      60611, United States.
LA  - eng
PT  - Journal Article
DEP - 20220121
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy, Large-Core Needle/methods/*statistics & numerical data
MH  - Female
MH  - Giant Cell Tumors/*diagnosis/pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Optical Imaging/methods/statistics & numerical data
OTO - NOTNLM
OT  - Core needle biopsy
OT  - Giant cell-rich bone tumors
EDAT- 2022/01/30 06:00
MHDA- 2022/03/29 06:00
CRDT- 2022/01/29 05:37
PHST- 2022/01/05 00:00 [received]
PHST- 2022/01/18 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/01/30 06:00 [pubmed]
PHST- 2022/03/29 06:00 [medline]
PHST- 2022/01/29 05:37 [entrez]
AID - S0344-0338(22)00020-6 [pii]
AID - 10.1016/j.prp.2022.153777 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2022 Mar;231:153777. doi: 10.1016/j.prp.2022.153777. Epub 2022 
      Jan 21.