PMID- 35091561 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220208 IS - 2058-7716 (Print) IS - 2058-7716 (Electronic) IS - 2058-7716 (Linking) VI - 8 IP - 1 DP - 2022 Jan 28 TI - LncRNA XR_596701 protects H9c2 cells against intermittent hypoxia-induced injury through regulation of the miR-344b-5p/FAIM3 axis. PG - 42 LID - 10.1038/s41420-022-00834-8 [doi] LID - 42 AB - Long noncoding RNAs (lncRNAs) participate in various biological processes and cardiovascular diseases. Recently, a novel lncRNA XR_596701 was found to be differentially expressed in obstructive sleep apnea (OSA)-induced myocardial tissue compared to normal myocardial tissues. However, the pathological effect and regulatory mechanism of XR_596701 in intermittent hypoxia (IH)-mediated cardiomyocytes damage have not been studied. The subcellular localization of XR_596701 was determined by fluorescence in situ hybridization (FISH). Gene expressions of XR_596701 and miR-344b-5p were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in IH-induced H9c2 cells. Cell proliferation was measured by 5-ethynyl-2'-deoxyuridine (EdU) staining assay. Cell apoptosis was detected by Hoechst 33342/PI staining and immunofluorescence (IF). Apoptotic protein of H9c2 cells was measured by western blot. The direct interaction between XR_596701 and miR-344b-5p as well as miR-344b-5p and Fas apoptotic inhibitory molecule 3 (FAIM3) were examined using dual-luciferase reporter assay. The significance of XR_596701 and miR-344b-5p on cell proliferation and apoptosis was evaluated by using gain-of-function and loss-of-function approaches. XR_596701 was upregulated, while miR-344b-5p downregulated in IH-induced H9c2 cells. Functionally, suppression of XR_596701 and overexpression of miR-344b-5p inhibited cell proliferation and promoted cell apoptosis in H9c2 cells. The roles of XR_596701 were achieved by sponging miR-344b-5p. And the function of miR-344b-5p was reversed by targeting FAIM3. Additionally, FAIM3 mediated IH-induced H9c2 cells damage by XR_596701. XR_596701 was serve as a novel lncRNA that indicated protective roles on proliferation and apoptosis of IH-induced H9c2 cells through the miR-344b-5p/FAIM3 axis. CI - (c) 2022. The Author(s). FAU - Chen, Qingshi AU - Chen Q AD - Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Fujian Medical University, No. 950 Donghai Street, Fengze District, Quanzhou, 362000, China. FAU - Lin, Guofu AU - Lin G AD - Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, No. 34 Zhongshan North Road, Licheng District, Quanzhou, 362000, China. FAU - Lin, Lanlan AU - Lin L AD - Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, No. 34 Zhongshan North Road, Licheng District, Quanzhou, 362000, China. FAU - Huang, Jiefeng AU - Huang J AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. FAU - Chen, Lida AU - Chen L AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. AD - Department of Respiratory and Critical Care Medicine, Zhangzhou Affiliated Hospital of Fujian Medical University, No. 59, Shenglixi Road, Xiangcheng District, Zhangzhou, 363000, China. FAU - Lian, Ningfang AU - Lian N AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. FAU - Chen, Mengxue AU - Chen M AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. FAU - Zeng, Aiming AU - Zeng A AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. FAU - Lin, Qichang AU - Lin Q AUID- ORCID: 0000-0003-3081-8084 AD - Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, 350005, China. chang4e@126.com. LA - eng GR - 81870074/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 82000094/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 2020J01203/Natural Science Foundation of Fujian Province (Fujian Provincial Natural Science Foundation)/ PT - Journal Article DEP - 20220128 PL - United States TA - Cell Death Discov JT - Cell death discovery JID - 101665035 PMC - PMC8799738 COIS- The authors declare no competing interests. EDAT- 2022/01/30 06:00 MHDA- 2022/01/30 06:01 PMCR- 2022/01/28 CRDT- 2022/01/29 05:44 PHST- 2021/09/12 00:00 [received] PHST- 2022/01/13 00:00 [accepted] PHST- 2021/12/31 00:00 [revised] PHST- 2022/01/29 05:44 [entrez] PHST- 2022/01/30 06:00 [pubmed] PHST- 2022/01/30 06:01 [medline] PHST- 2022/01/28 00:00 [pmc-release] AID - 10.1038/s41420-022-00834-8 [pii] AID - 834 [pii] AID - 10.1038/s41420-022-00834-8 [doi] PST - epublish SO - Cell Death Discov. 2022 Jan 28;8(1):42. doi: 10.1038/s41420-022-00834-8.