PMID- 35092082
OWN - NLM
STAT- MEDLINE
DCOM- 20220330
LR  - 20220531
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 40
IP  - 2
DP  - 2022 Mar
TI  - Vanillin attenuates thioacetamide-induced renal assault by direct and indirect 
      mediation of the TGFbeta, ERK and Smad signalling pathways in rats.
PG  - 175-188
LID - 10.1002/cbf.3686 [doi]
AB  - Inflammation and fibrosis are two pathological features of chronic kidney disease 
      (CKD). Renal fibrosis is considered to be one of the most important conditions, 
      as it may be the result of excessive extracellular matrix protein production and 
      deposition, or prolonged exposure to nephrotoxic substances or drugs. 
      Unfortunately, no suitable therapies or medications are currently available to 
      prevent renal fibrosis. We conducted this study for the evaluation of the 
      protective potential of vanillin by reversing TAA (250 mg/kg TAA for 6 weeks) 
      induced renal injury in rats. The concentrations of the proteins tumour necrosis 
      factor alpha (TNFalpha), interleukin-6 (IL-6), extracellular signal regulated kinase 
      1/2 (Erk1/2), and transforming growth factor beta-1 (TGF-beta1) in kidney tissues 
      were assessed using ELISA. Kidney Injury Molecule-1 (KIM-1) and mothers against 
      decapentaplegic homologue 2, 3 (SMAD 2, 3) expressions were evaluated using real 
      time PCR. We also estimated the expression of alpha-smooth muscle actin (alpha-SMA) using 
      immunohistochemistry. Treatment with vanillin (100 mg/kg) significantly 
      ameliorated kidney Injury and improved the kidney function. Vanillin treatment 
      also significantly decreased the malondialdehyde (MDA) content, and elevated 
      glutathione peroxidase (GPx) and catalase (CAT) activities in kidney tissues. 
      Vanillin also reduced alpha-SMA renal expression and TNFalpha, IL-6, TGF-beta1, and Erk1/2 
      renal levels. Vanillin significantly decreased the expression of the genes 
      encoding KIM-1 and SMAD 2, 3 and ameliorated histological abnormalities in kidney 
      architecture. Our molecular docking findings showed that vanillin has a good 
      binding mode inside TGF-beta type I receptors (ALK5) biding site.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Metwaly, Heba A
AU  - Metwaly HA
AUID- ORCID: 0000-0002-9593-4687
AD  - Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Alexandria 
      University, Alexandria, Egypt.
AD  - Department of Biochemistry, Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - El-Eraky, Abdulrahman M
AU  - El-Eraky AM
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Ibrahim, Eman E
AU  - Ibrahim EE
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Kandil, Khaled K
AU  - Kandil KK
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - El-Sayed, Mohamed A
AU  - El-Sayed MA
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - El-Tabakh, Nayra M
AU  - El-Tabakh NM
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Motawea, Amr M
AU  - Motawea AM
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Ali, Helmi A
AU  - Ali HA
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Jabban, Maher Z
AU  - Jabban MZ
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Mahmoud, Mona E
AU  - Mahmoud ME
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Abdelfattah, Walaa H
AU  - Abdelfattah WH
AD  - Faculty of Pharmacy, Delta University, Gamasa, Egypt.
FAU - Elmorsy, Mohammad A
AU  - Elmorsy MA
AD  - Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Mansoura 
      University, Mansoura, Egypt.
FAU - Ghanim, Amal M H
AU  - Ghanim AMH
AD  - Department of Biochemistry, Faculty of Pharmacy, Fayoum University, Fayoum, 
      Egypt.
LA  - eng
PT  - Journal Article
DEP - 20220129
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Benzaldehydes)
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 075T165X8M (Thioacetamide)
RN  - CHI530446X (vanillin)
SB  - IM
MH  - Animals
MH  - *Benzaldehydes/pharmacology
MH  - Fibrosis
MH  - *Kidney/drug effects/metabolism/pathology
MH  - MAP Kinase Signaling System/drug effects
MH  - Molecular Docking Simulation
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - *Smad Proteins/metabolism
MH  - *Thioacetamide/antagonists & inhibitors/toxicity
MH  - *Transforming Growth Factor beta1/metabolism
OTO - NOTNLM
OT  - ALK5
OT  - Erk1/2
OT  - IL-6
OT  - SMAD
OT  - TGF-beta1
OT  - vanillin
EDAT- 2022/01/30 06:00
MHDA- 2022/03/31 06:00
CRDT- 2022/01/29 05:47
PHST- 2021/10/18 00:00 [revised]
PHST- 2021/08/29 00:00 [received]
PHST- 2021/10/28 00:00 [accepted]
PHST- 2022/01/30 06:00 [pubmed]
PHST- 2022/03/31 06:00 [medline]
PHST- 2022/01/29 05:47 [entrez]
AID - 10.1002/cbf.3686 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2022 Mar;40(2):175-188. doi: 10.1002/cbf.3686. Epub 2022 Jan 
      29.