PMID- 35093500
OWN - NLM
STAT- MEDLINE
DCOM- 20220506
LR  - 20220506
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 142
DP  - 2022 Mar
TI  - Doublesex regulates male-specific differentiation during distinct developmental 
      time windows in a parasitoid wasp.
PG  - 103724
LID - S0965-1748(22)00006-6 [pii]
LID - 10.1016/j.ibmb.2022.103724 [doi]
AB  - Sexually dimorphic traits in insects are subject to sexual selection, but our 
      knowledge of the underlying molecular mechanisms is still scarce. Here we 
      investigate how the highly conserved gene, Doublesex (Dsx), is involved in 
      shaping sexual dimorphism in the model parasitoid wasp Nasonia vitripennis 
      (Hymenoptera: Pteromalidae). First, we present the revised Dsx gene structure 
      including an alternative transcription start, and two additional male NvDsx 
      transcript isoforms. We show sex-specific NvDsx expression and splicing 
      throughout development, and demonstrate that transient NvDsx silencing in 
      different male developmental stages shifts two sexually dimorphic traits from 
      male to female morphology, with the effect being dependent on the timing of 
      silencing. In addition, we determined the effect of NvDsx on the development of 
      reproductive organs. Transient silencing of NvDsx in early male larvae affects 
      the growth and differentiation of the internal and external reproductive tissues. 
      We did not observe phenotypic changes in females after NvDsx silencing. Our 
      results indicate that male NvDsx is required to suppress female-specific traits 
      and/or to promote male-specific traits during distinct developmental windows. 
      This provides new insights into the regulatory activity of Dsx during male wasp 
      development in the Hymenoptera.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Wang, Yidong
AU  - Wang Y
AD  - Wageningen University, Laboratory of Entomology, Wageningen, the Netherlands.
FAU - Rensink, Anna H
AU  - Rensink AH
AD  - Evolutionary Genetics, Development and Behaviour, Groningen Institute for 
      Evolutionary Life Sciences, University of Groningen, Groningen, the Netherlands.
FAU - Fricke, Ute
AU  - Fricke U
AD  - Wageningen University, Laboratory of Entomology, Wageningen, the Netherlands.
FAU - Riddle, Megan C
AU  - Riddle MC
AD  - Biology Department, Western Washington University, Washington, USA.
FAU - Trent, Carol
AU  - Trent C
AD  - Biology Department, Western Washington University, Washington, USA.
FAU - van de Zande, Louis
AU  - van de Zande L
AD  - Evolutionary Genetics, Development and Behaviour, Groningen Institute for 
      Evolutionary Life Sciences, University of Groningen, Groningen, the Netherlands.
FAU - Verhulst, Eveline C
AU  - Verhulst EC
AD  - Wageningen University, Laboratory of Entomology, Wageningen, the Netherlands; 
      Wageningen University, Laboratory of Genetics, Wageningen, the Netherlands. 
      Electronic address: eveline.verhulst@wur.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220129
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Female
MH  - Larva/genetics
MH  - Male
MH  - Protein Isoforms/genetics
MH  - RNA Splicing
MH  - Sex Characteristics
MH  - *Wasps/genetics
OTO - NOTNLM
OT  - Doublesex
OT  - Nasonia
OT  - Parasitoid wasps
OT  - Sex determination
OT  - Sexual differentiation
OT  - Sexually dimorphic traits
EDAT- 2022/01/31 06:00
MHDA- 2022/05/07 06:00
CRDT- 2022/01/30 20:29
PHST- 2021/09/13 00:00 [received]
PHST- 2022/01/19 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/01/31 06:00 [pubmed]
PHST- 2022/05/07 06:00 [medline]
PHST- 2022/01/30 20:29 [entrez]
AID - S0965-1748(22)00006-6 [pii]
AID - 10.1016/j.ibmb.2022.103724 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2022 Mar;142:103724. doi: 10.1016/j.ibmb.2022.103724. 
      Epub 2022 Jan 29.