PMID- 35093775
OWN - NLM
STAT- MEDLINE
DCOM- 20220221
LR  - 20220221
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 241
DP  - 2022 May 1
TI  - A controlled recognizing and releasing glycoprotein based on 
      temperature-responsive phenylboronic microgels for colorimetric analysis of 
      complex samples.
PG  - 123260
LID - S0039-9140(22)00056-X [pii]
LID - 10.1016/j.talanta.2022.123260 [doi]
AB  - It is challenging to capture glycoprotein from complicated samples with both high 
      specificity and high recovery. Herein, we synthesized temperature-responsive poly 
      (N-isopropylacrylamide-4-vinylbenzeneboronic acid) (PNIPAM-VPBA) microgels having 
      enhanced affinity capability towards glycoprotein, resulting signal amplification 
      in colorimetric analysis subsequently. Through temperature control, the target 
      glycoprotein can be captured and released by the reported microgels unbiasedly. 
      The microgels fabricated were characterized in terms of particle size, phase 
      transition temperature, and toxicity. It was revealed that obvious changes in the 
      particle diameter ranged from 600 to 323 nm between 25  degrees C and 55  degrees C, the volume 
      transition temperature was close to human physiological temperature, and low 
      toxicity towards HeLa cells after 24 h incubation. Using glucose oxidase as a 
      model target, the microgels were applied as adsorbent materials for 
      preconcentration of glucose oxidase, resulting 22-fold signal enhancement and a 
      limit of detection (LOD) as low as 16.7 U/L. In beer sample analysis, satisfied 
      recoveries of 78.7%-101.6% with the relative standard deviations (RSDs) less than 
      7.2% was observed. Subsequently, the microgels were combined with enzyme-linked 
      immunosorbent assay (ELISA) for alpha fetoprotein (AFP) determination, by 
      immobilizing horseradish peroxidase (HRP) and anti-AFP antibodies on the surface 
      of microgels. A meaningful low concentration range of 2.5 x 10(-2) to 1.0 mug/L 
      was obtained for AFP detection using the microgels based colorimetric method with 
      a LOD of 8.4 ng/L. In human serum sample analysis, good accuracy was achieved 
      from method comparing with commercial ELISA kit, satisfied recoveries of 
      86.1%-96.4% with the RSDs less than 6.0% was observed. The proposed PNIPAM-VPBA 
      microgels have great potential applications in the fields of food and clinical 
      analysis.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Su, Rihui
AU  - Su R
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Xia, Ling
AU  - Xia L
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Li, Gongke
AU  - Li G
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China. Electronic 
      address: cesgkl@mail.sysu.edu.cn.
FAU - Xiao, Xiaohua
AU  - Xiao X
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China. Electronic 
      address: xiaoxhua@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220124
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Gels)
RN  - 0 (Glycoproteins)
RN  - 0 (Microgels)
SB  - IM
MH  - Colorimetry
MH  - Gels
MH  - Glycoproteins
MH  - HeLa Cells
MH  - Humans
MH  - *Microgels
MH  - Temperature
OTO - NOTNLM
OT  - Enzyme-linked immunosorbent assay
OT  - Glycoprotein
OT  - PNIPAM-VPBA microgels
OT  - Phenylboronic acid
EDAT- 2022/01/31 06:00
MHDA- 2022/02/22 06:00
CRDT- 2022/01/30 20:33
PHST- 2022/01/02 00:00 [received]
PHST- 2022/01/15 00:00 [revised]
PHST- 2022/01/22 00:00 [accepted]
PHST- 2022/01/31 06:00 [pubmed]
PHST- 2022/02/22 06:00 [medline]
PHST- 2022/01/30 20:33 [entrez]
AID - S0039-9140(22)00056-X [pii]
AID - 10.1016/j.talanta.2022.123260 [doi]
PST - ppublish
SO  - Talanta. 2022 May 1;241:123260. doi: 10.1016/j.talanta.2022.123260. Epub 2022 Jan 
      24.