PMID- 35095965
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220201
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. 
      Subsoil of Three Grassland Habitats in Northern China.
PG  - 792002
LID - 10.3389/fpls.2021.792002 [doi]
LID - 792002
AB  - The microbial groups of nitrogen fixers, ammonia oxidizers, and denitrifiers play 
      vital roles in driving the nitrogen cycle in grassland ecosystems. However, the 
      understanding of the abundance and distribution of these functional 
      microorganisms as well as their driving factors were limited mainly to topsoil. 
      In this study, the abundances of nitrogen functional genes (NFGs) involved in 
      nitrogen fixation (nifH), ammonia oxidation (amoA), and denitrification (nirK, 
      nirS, and nosZ) were investigated in both topsoil (0-10 cm, soil layer with 
      concentrated root) and subsoil (30-40 cm, soil layer with spare root) of three 
      grassland habitats in northern China. The abundance of NFGs decreased with soil 
      depth except for the archaeal amoA gene and the distribution of nifH, archaeal 
      amoA, nirK, and nirS gene was significantly impacted by grassland habitats. 
      Moreover, the distribution of NFGs was more responsive to the vertical difference 
      than horizontal spatial heterogeneity. Redundancy analysis revealed that the 
      distribution pattern of overall NFGs was regulated by grassland habitats, and 
      these regulations were more obvious in the subsoil than in the topsoil. Variance 
      partitioning analysis further indicated that soil resource supply (e.g., organic 
      matter) may control the vertical distribution of NFGs. Taken together, the 
      findings in this study could fundamentally improve our understanding of the 
      distribution of N cycling-associated microorganisms across a vertical scale, 
      which would be useful for predicting the soil N availability and guiding the soil 
      N management in grassland ecosystems.
CI  - Copyright (c) 2022 Liu, Chi, Cheng, Ding, Wen, Zhao, Feng, Zhang, Cai and Liu.
FAU - Liu, Yuqing
AU  - Liu Y
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences (CAS), Beijing, China.
AD  - College of Resources and Environment, University of Chinese Academy of Sciences 
      (CAS), Beijing, China.
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
FAU - Chi, Qiaodong
AU  - Chi Q
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
FAU - Cheng, Hui
AU  - Cheng H
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
FAU - Ding, Huanxin
AU  - Ding H
AD  - Suzhou Station of Farmland Quality Protection, Suzhou, China.
FAU - Wen, Teng
AU  - Wen T
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
FAU - Zhao, Jun
AU  - Zhao J
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
AD  - Jiangsu Center for Collaborative Innovation in Geographical Information Resource 
      Development and Application, Nanjing, China.
AD  - Zhongke Clean Soil (Guangzhou) Technology Service Co., Ltd., Guangzhou, China.
FAU - Feng, Xiaojuan
AU  - Feng X
AD  - State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, 
      Chinese Academy of Sciences (CAS), Beijing, China.
FAU - Zhang, Jinbo
AU  - Zhang J
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
AD  - Jiangsu Center for Collaborative Innovation in Geographical Information Resource 
      Development and Application, Nanjing, China.
AD  - Zhongke Clean Soil (Guangzhou) Technology Service Co., Ltd., Guangzhou, China.
FAU - Cai, Zucong
AU  - Cai Z
AD  - School of Geography, Nanjing Normal University, Nanjing, China.
AD  - Zhongke Clean Soil (Guangzhou) Technology Service Co., Ltd., Guangzhou, China.
AD  - Key Laboratory of Virtual Geographical Environment, Ministry of Education, 
      Nanjing Normal University, Nanjing, China.
FAU - Liu, Guohua
AU  - Liu G
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences (CAS), Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20220114
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8798409
OTO - NOTNLM
OT  - abundance
OT  - grassland ecosystem
OT  - nitrogen functional gene
OT  - real-time PCR
OT  - soil depth
COIS- JZ, JBZ, and ZC were employed by the company Zhongke Clean Soil (Guangzhou) 
      Technology Service Co., Ltd. The remaining authors declare that the research was 
      conducted in the absence of any commercial or financial relationships that could 
      be construed as a potential conflict of interest.
EDAT- 2022/02/01 06:00
MHDA- 2022/02/01 06:01
PMCR- 2021/01/01
CRDT- 2022/01/31 05:57
PHST- 2021/10/09 00:00 [received]
PHST- 2021/12/09 00:00 [accepted]
PHST- 2022/01/31 05:57 [entrez]
PHST- 2022/02/01 06:00 [pubmed]
PHST- 2022/02/01 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.792002 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Jan 14;12:792002. doi: 10.3389/fpls.2021.792002. 
      eCollection 2021.