PMID- 35096319
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220531
IS  - 2008-9872 (Electronic)
IS  - 0365-3439 (Print)
IS  - 0365-3439 (Linking)
VI  - 76
IP  - 4
DP  - 2021 Oct
TI  - Sequence Analysis of Transcription Factor 7-like 2 Protein (TCF7L2) in Iraqi 
      Patients with Diabetic Mellitus Type 2 Using Bioinformatics Methods.
PG  - 841-846
LID - 10.22092/ari.2021.355851.1729 [doi]
AB  - Several previously published reports have suggested a relationship between type 2 
      diabetes mellitus (T2DM) and chromosome 10q. The results of genotyping of 228 
      microsatellite markers in Icelandic people with T2DMrevealed that a 
      microsatellite, DG10S478, within intron 3 of the transcription factor 7-like 2 
      gene (TCF7L2; formerly TCF4) was associated with T2DM. The present study was 
      aimed to analyze the sequence of TCF7L2 in Iraqi patients with T2DM. This study 
      was performed on the blood samples of 10 patients within the age range of 18-70 
      years old with T2DM. The DNA was extracted from the whole blood samples and the 
      TCF7L2 gene was purified and amplified using the polymerase chain reaction (PCR) 
      technique. Afterward, the PCR products were run in gel electrophoresis to detect 
      the gene. Moreover, the BLAST software was used to analyze the gene TCF7L2 
      sequence which was compared with the reference sequence of the template gene from 
      NCBI. The results of TCF7L2 gene sequences obtained from the samples collected 
      from the Iraqi patients with T2DMwere received from Macogen Company, Korea, and 
      analyzed using the BLAST software. The findings showed mutations in the gene 
      sequence of all patients, compared to the gene sequences in NCBI. Hence, the 
      mutation in the TCF7L2 gene was present in Iraqi patients with T2DM, and it could 
      be one of the factors causing and increasing the risk of T2DM disease.
FAU - Kareem Queen, B
AU  - Kareem Queen B
AD  - Department of Applied Sciences, University of Technology, Baghdad, Iraq.
FAU - Abdullah Al-Janabi, A
AU  - Abdullah Al-Janabi A
AD  - Department of Applied Sciences, University of Technology, Baghdad, Iraq.
LA  - eng
PT  - Journal Article
DEP - 20211031
PL  - Iran
TA  - Arch Razi Inst
JT  - Archives of Razi Institute
JID - 101549567
RN  - 0 (TCF7L2 protein, human)
RN  - 0 (Transcription Factor 7-Like 2 Protein)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Computational Biology
MH  - *Diabetes Mellitus, Type 2/genetics
MH  - Humans
MH  - Iraq
MH  - Middle Aged
MH  - Sequence Analysis
MH  - *Transcription Factor 7-Like 2 Protein/genetics
MH  - Young Adult
PMC - PMC8790997
OTO - NOTNLM
OT  - Bioinformatics Methods
OT  - Diabetic mellitus type 2
OT  - Mutation
OT  - TCF7L2 gene
EDAT- 2022/02/01 06:00
MHDA- 2022/03/16 06:00
PMCR- 2021/10/31
CRDT- 2022/01/31 05:58
PHST- 2021/08/27 00:00 [received]
PHST- 2021/09/14 00:00 [accepted]
PHST- 2022/01/31 05:58 [entrez]
PHST- 2022/02/01 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2021/10/31 00:00 [pmc-release]
AID - ARI-76-4 [pii]
AID - 10.22092/ari.2021.355851.1729 [doi]
PST - epublish
SO  - Arch Razi Inst. 2021 Oct 31;76(4):841-846. doi: 10.22092/ari.2021.355851.1729. 
      eCollection 2021 Oct.