PMID- 35099787
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2417
DP  - 2022
TI  - Real-Time Quantitative PCR and Fluorescence In Situ Hybridization for Subcellular 
      Localization of miRNAs in Neurons.
PG  - 1-17
LID - 10.1007/978-1-0716-1916-2_1 [doi]
AB  - Neuronal miRNAs play major roles in regulation of synaptic development and 
      plasticity. The small size of miRNAs and, in some cases, their low level of 
      expression make their quantification and detection challenging. Here, we outline 
      methods to quantify steady state levels of miRNAs in neurons and the brain by 
      using real-time quantitative PCR (RT-qPCR) and to determine miRNA subcellular 
      localization in primary neurons by a sensitive fluorescence in situ hybridization 
      (FISH) method.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Tatarakis, Antonis
AU  - Tatarakis A
AD  - Department of Cell Biology, and Howard Hughes Medical Institute, Harvard Medical 
      School, Boston, MA, USA. antonis_tatarakis@hms.harvard.edu.
FAU - Moazed, Danesh
AU  - Moazed D
AD  - Department of Cell Biology, and Howard Hughes Medical Institute, Harvard Medical 
      School, Boston, MA, USA. danesh@hms.harvard.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (MicroRNAs)
SB  - IM
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/metabolism
MH  - Neurons/metabolism
MH  - Real-Time Polymerase Chain Reaction/methods
OTO - NOTNLM
OT  - Brain
OT  - Expression profile
OT  - Isolation
OT  - Neurons
OT  - miRNA RT-qPCR assays
OT  - miRNA fluorescence in situ hybridization
OT  - miRNAs
EDAT- 2022/02/01 06:00
MHDA- 2022/04/01 06:00
CRDT- 2022/01/31 12:18
PHST- 2022/01/31 12:18 [entrez]
PHST- 2022/02/01 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
AID - 10.1007/978-1-0716-1916-2_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2417:1-17. doi: 10.1007/978-1-0716-1916-2_1.