PMID- 35106541
OWN - NLM
STAT- MEDLINE
DCOM- 20220307
LR  - 20230202
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 163
IP  - 4
DP  - 2022 Apr 1
TI  - PCP Protein Inversin Regulates Testis Function Through Changes in Cytoskeletal 
      Organization of Actin and Microtubules.
LID - 10.1210/endocr/bqac009 [doi]
LID - bqac009
AB  - Inversin is an integrated component of the Frizzled (Fzd)/Dishevelled 
      (Dvl)/Diversin planar cell polarity (PCP) complex that is known to work in 
      concert with the Van Gogh-like protein (eg, Vangl2)/Prickle PCP complex to 
      support tissue and organ development including the brain, kidney, pancreas, and 
      others. These PCP protein complexes are also recently shown to confer developing 
      haploid spermatid PCP to support spermatogenesis in adult rat testes. However, 
      with the exception of Dvl3 and Vangl2, other PCP proteins have not been 
      investigated in the testis. Herein, we used the technique of RNA interference 
      (RNAi) to examine the role of inversin (Invs) in Sertoli cell (SC) and testis 
      function by corresponding studies in vitro and in vivo. When inversin was 
      silenced by RNAi using specific small interfering RNA duplexes by transfecting 
      primary cultures of SCs in vitro or testes in vivo, it was shown that inversin 
      knockdown (KD) perturbed the SC tight junction-barrier function in vitro and in 
      vivo using corresponding physiological and integrity assays. More important, 
      inversin exerted its regulatory effects through changes in the organization of 
      the actin and microtubule cytoskeletons, including reducing the ability of their 
      polymerization. These changes, in turn, induced defects in spermatogenesis by 
      loss of spermatid polarity, disruptive distribution of blood-testis 
      barrier-associated proteins at the SC-cell interface, appearance of 
      multinucleated round spermatids, and defects in the release of sperm at 
      spermiation.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the 
      Endocrine Society. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Li, Linxi
AU  - Li L
AUID- ORCID: 0000-0003-4021-4102
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Gao, Sheng
AU  - Gao S
AD  - Institute of Reproductive Medicine, Nantong University School of Medicine, 
      Nantong, Jiangsu 226001, China.
FAU - Wang, Lingling
AU  - Wang L
AD  - Institute of Reproductive Medicine, Nantong University School of Medicine, 
      Nantong, Jiangsu 226001, China.
FAU - Bu, Tiao
AU  - Bu T
AD  - Institute of Reproductive Medicine, Nantong University School of Medicine, 
      Nantong, Jiangsu 226001, China.
FAU - Chu, Jinjin
AU  - Chu J
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Lv, Lixiu
AU  - Lv L
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Tahir, Anam
AU  - Tahir A
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Mao, Baiping
AU  - Mao B
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Li, Huitao
AU  - Li H
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Li, Xiaoheng
AU  - Li X
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Wang, Yiyan
AU  - Wang Y
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Wu, Xiaolong
AU  - Wu X
AUID- ORCID: 0000-0003-3050-0324
AD  - Institute of Reproductive Medicine, Nantong University School of Medicine, 
      Nantong, Jiangsu 226001, China.
FAU - Ge, Renshan
AU  - Ge R
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
FAU - Cheng, C Yan
AU  - Cheng CY
AUID- ORCID: 0000-0003-3117-3791
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325027, China.
AD  - Institute of Reproductive Medicine, Nantong University School of Medicine, 
      Nantong, Jiangsu 226001, China.
AD  - Department of Urology and Andrology, Sir Run Run Shaw Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang 310016, China.
LA  - eng
GR  - R01 HD056034/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Actins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Actins/*metabolism
MH  - Animals
MH  - Blood-Testis Barrier/metabolism
MH  - Cytoskeleton/*metabolism
MH  - Male
MH  - Microtubules/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sertoli Cells/metabolism
MH  - Spermatids/metabolism
MH  - Spermatogenesis/physiology
MH  - Testis/*metabolism
MH  - Transcription Factors/*metabolism
PMC - PMC8870424
OTO - NOTNLM
OT  - Sertoli cells
OT  - inversin
OT  - planar cell polarity
OT  - spermatogenesis
OT  - testis
EDAT- 2022/02/03 06:00
MHDA- 2022/03/08 06:00
PMCR- 2023/02/01
CRDT- 2022/02/02 05:35
PHST- 2021/11/24 00:00 [received]
PHST- 2022/02/03 06:00 [pubmed]
PHST- 2022/03/08 06:00 [medline]
PHST- 2022/02/02 05:35 [entrez]
PHST- 2023/02/01 00:00 [pmc-release]
AID - 6519617 [pii]
AID - bqac009 [pii]
AID - 10.1210/endocr/bqac009 [doi]
PST - ppublish
SO  - Endocrinology. 2022 Apr 1;163(4):bqac009. doi: 10.1210/endocr/bqac009.