PMID- 35106909
OWN - NLM
STAT- MEDLINE
DCOM- 20220420
LR  - 20220420
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 26
IP  - 5
DP  - 2022 Mar
TI  - Polycystin-1 and hydrostatic pressure are implicated in glioblastoma pathogenesis 
      in vitro.
PG  - 1699-1709
LID - 10.1111/jcmm.17212 [doi]
AB  - The mechanobiological aspects of glioblastoma (GBM) pathogenesis are largely 
      unknown. Polycystin-1 (PC1) is a key mechanosensitive protein which perceives 
      extracellular mechanical cues and transforms them into intracellular biochemical 
      signals that elicit a change in cell behaviour. The aim of the present study was 
      to investigate if and how PC1 participates in GBM pathogenesis under a 
      mechanically induced microenvironment. Therefore, we subjected T98G GBM cells to 
      continuous hydrostatic pressure (HP) and/or PC1 blockade and evaluated their 
      effect on cell behaviour, the activity of signalling pathways and the expression 
      of mechano-induced transcriptional regulators and markers associated with 
      properties of cancer cells. According to our data, PC1 and HP affect GBM cell 
      proliferation, clonogenicity and migration; the diameter of GBM spheroids; the 
      phosphorylation of mechanistic target of rapamycin (mTOR), extracellular 
      signal-regulated kinase (ERK) and focal adhesion kinase (FAK); the protein 
      expression of transcription cofactors YES-associated protein (YAP) and 
      transcriptional coactivator with PDZ-binding motif (TAZ); and the mRNA expression 
      of markers related to anti-apoptosis, apoptosis, angiogenesis, epithelial to 
      mesenchymal transition (EMT) and proliferation. Together, our in vitro results 
      suggest that PC1 plays an important role in GBM mechanobiology.
CI  - (c) 2022 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Zoi, Ilianna
AU  - Zoi I
AUID- ORCID: 0000-0002-0435-2463
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
FAU - Gargalionis, Antonios N
AU  - Gargalionis AN
AUID- ORCID: 0000-0003-4332-6409
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
AD  - Department of Biopathology, 'Aeginition' Hospital, Medical School, National and 
      Kapodistrian University of Athens, Athens, Greece.
FAU - Papavassiliou, Kostas A
AU  - Papavassiliou KA
AUID- ORCID: 0000-0001-9620-745X
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
FAU - Nasiri-Ansari, Narjes
AU  - Nasiri-Ansari N
AUID- ORCID: 0000-0002-0116-693X
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
FAU - Piperi, Christina
AU  - Piperi C
AUID- ORCID: 0000-0002-2701-0618
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
FAU - Basdra, Efthimia K
AU  - Basdra EK
AUID- ORCID: 0000-0002-0913-9354
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
FAU - Papavassiliou, Athanasios G
AU  - Papavassiliou AG
AUID- ORCID: 0000-0001-5803-4527
AD  - Department of Biological Chemistry, Medical School, National and Kapodistrian 
      University of Athens, Athens, Greece.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220201
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (TRPP Cation Channels)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - *Glioblastoma/pathology
MH  - Humans
MH  - Hydrostatic Pressure
MH  - TRPP Cation Channels/genetics/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Tumor Microenvironment
PMC - PMC8899169
OTO - NOTNLM
OT  - glioblastoma
OT  - hydrostatic pressure
OT  - mechanobiology
OT  - polycystin-1
COIS- The authors confirm that there are no conflicts of interest.
EDAT- 2022/02/03 06:00
MHDA- 2022/04/21 06:00
PMCR- 2022/03/01
CRDT- 2022/02/02 05:39
PHST- 2022/01/20 00:00 [revised]
PHST- 2022/01/04 00:00 [received]
PHST- 2022/01/22 00:00 [accepted]
PHST- 2022/02/03 06:00 [pubmed]
PHST- 2022/04/21 06:00 [medline]
PHST- 2022/02/02 05:39 [entrez]
PHST- 2022/03/01 00:00 [pmc-release]
AID - JCMM17212 [pii]
AID - 10.1111/jcmm.17212 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2022 Mar;26(5):1699-1709. doi: 10.1111/jcmm.17212. Epub 2022 Feb 
      1.