PMID- 3510871
OWN - NLM
STAT- MEDLINE
DCOM- 19860312
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 154
IP  - 2
DP  - 1986 Jan 15
TI  - The phosphoenolpyruvate:glucose phosphotransferase system of Salmonella 
      typhimurium. The phosphorylated form of IIIGlc.
PG  - 337-41
AB  - Enzyme IIIGlc of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) 
      of Salmonella typhimurium can occur in two forms: phosphorylated and 
      nonphosphorylated. Phosphorylated IIIGlc (P-IIIGlc) has a slightly lower mobility 
      during sodium dodecyl sulphate/polyacrylamide gel electrophoresis than IIIGlc. In 
      bacterial extracts both phosphoenolpyruvate (the physiological phosphoryl donor 
      of the PTS) as well as ATP can phosphorylate IIIGlc. The ATP-catalyzed reaction 
      is dependent on phosphoenolpyruvate synthase, however, and is due to prior 
      conversion of ATP to phosphoenolpyruvate. The phosphoryl group of phosphorylated 
      IIIGlc is hydrolysed after boiling in sodium dodecyl sulfate but phosphorylated 
      IIIGlc can be discriminated from IIIGlc if treated with this detergent at room 
      temperature. We have used the different mobilities of IIIGlc and P-IIIGlc to 
      estimate the proportion of these two forms in intact cells. Wild-type cells 
      contain predominantly P-IIIGlc in the absence of PTS sugars. In an S. typhimurium 
      mutant containing a leaky ptsI17 mutation (0.1% enzyme I activity remaining) both 
      forms of IIIGlc occur in approximately equal amounts. Addition of PTS sugars such 
      as glucose results, both in wild-type and mutant, in a dephosphorylation of 
      P-IIIGlc. This correlates well with the observed inhibition of non-PTS uptake 
      systems by PTS sugars via nonphosphorylated IIIGlc.
FAU - Nelson, S O
AU  - Nelson SO
FAU - Schuitema, A R
AU  - Schuitema AR
FAU - Postma, P W
AU  - Postma PW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (crr protein, E coli)
RN  - EC 2.7.1.- (Phosphoenolpyruvate Sugar Phosphotransferase System)
SB  - IM
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli Proteins
MH  - Phosphoenolpyruvate Sugar Phosphotransferase System/*isolation & 
      purification/metabolism
MH  - Phosphorylation
MH  - Salmonella typhimurium/*enzymology
EDAT- 1986/01/15 00:00
MHDA- 1986/01/15 00:01
CRDT- 1986/01/15 00:00
PHST- 1986/01/15 00:00 [pubmed]
PHST- 1986/01/15 00:01 [medline]
PHST- 1986/01/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1986.tb09402.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1986 Jan 15;154(2):337-41. doi: 
      10.1111/j.1432-1033.1986.tb09402.x.