PMID- 35109967
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220531
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 34
IP  - 3
DP  - 2022 Feb
TI  - Interrelationships between amphiregulin, kisspeptin, FSH and FSH receptor in 
      promotion of human ovarian cell functions.
PG  - 362-377
LID - 10.1071/RD21230 [doi]
AB  - The aim of this study was to investigate: (1) the ability of granulosa cells to 
      produce amphiregulin (AREG), kisspeptin (KISS) and FSH receptor (FSHR); (2) the 
      role of AREG and KISS in the control of ovarian functions; (3) the effect of FSH 
      and KISS on AREG; and (4) the ability of KISS to affect FSHR and to modify FSH 
      action on AREG output by human ovarian granulosa cells. We examined: (1) 
      time-dependent accumulation of AREG; (2) effects of AREG (0, 1, 10, 100ng/mL) and 
      KISS (0, 1, 10, 100ng/mL) on granulosa cell functions; and (3) the effects of 
      KISS (0, 1, 10, 100ng/mL), FSH (0, 1, 10, 100ng/mL), and their combinations on 
      AREG release. Viability, markers of proliferation [accumulation ofproliferating 
      cell nuclear antigen (PCNA) cyclin B1 and sodium 
      3'-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy6-nitro)benzene 
      sulfonic acid hydrate (XTT formazan)] and apoptosis (accumulation of bax, caspase 
      3 and terminal deoxynucleotidyl transferase dUTP nick-end labelling), 
      accumulation of KISS, FSHR and steroid hormones, and AREG release were analysed 
      by Trypan blue exclusion test, quantitative immunocytochemistry, XTT, terminal 
      deoxynucleotidyl transferase dUTP nick-end labelling assays and enzyme-linked 
      immunosorbent assay. AREG promoted cell viability, proliferation and steroid 
      hormone output, and inhibited apoptosis. KISS (1 and 10ng/mL) stimulated 
      viability, proliferation, steroid hormone release and occurrence of FSHR and 
      suppressed apoptosis and AREG output; KISS (100ng/mL) had the opposite effect. 
      FSH stimulated AREG release, whilst addition of KISS reversed this FSH effect. 
      FSH mimicked and promoted the inhibitory effect of KISS on AREG release. These 
      results suggest an intra-ovarian production and a functional interrelationship 
      between AREG, KISS, FSH and FSHR in direct regulation of basic ovarian cell 
      functions.
FAU - Fabova, Zuzana
AU  - Fabova Z
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Tr. A. Hlinku 1, 94974 Nitra, Slovakia.
FAU - Loncova, Barbora
AU  - Loncova B
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Tr. A. Hlinku 1, 94974 Nitra, Slovakia.
FAU - Mlyn Ek, Milos
AU  - Mlyn Ek M
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Tr. A. Hlinku 1, 94974 Nitra, Slovakia.
FAU - Sirotkin, Alexander V
AU  - Sirotkin AV
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Tr. A. Hlinku 1, 94974 Nitra, Slovakia.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (FSHR protein, human)
RN  - 0 (Follicle Stimulating Hormone, Human)
RN  - 0 (KISS1 protein, human)
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, FSH)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Amphiregulin/*metabolism/pharmacology
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Follicle Stimulating Hormone, Human/*metabolism
MH  - Granulosa Cells
MH  - Humans
MH  - Kisspeptins/*metabolism/pharmacology
MH  - Ovary/*cytology/*physiology
MH  - Receptors, FSH/*metabolism
EDAT- 2022/02/04 06:00
MHDA- 2022/03/17 06:00
CRDT- 2022/02/03 05:28
PHST- 2021/08/17 00:00 [received]
PHST- 2021/11/16 00:00 [accepted]
PHST- 2022/02/04 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
PHST- 2022/02/03 05:28 [entrez]
AID - RD21230 [pii]
AID - 10.1071/RD21230 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2022 Feb;34(3):362-377. doi: 10.1071/RD21230.