PMID- 35113007
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20230204
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 21
IP  - 7
DP  - 2022 Apr
TI  - Long non-coding RNA AGAP2-AS1 promotes cell proliferation and invasion through 
      regulating miR-193a-3p/LOXL4 axis in laryngeal squamous cell carcinoma.
PG  - 697-707
LID - 10.1080/15384101.2021.2016197 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is an aggressive malignancy with highly 
      mortality rate. Long non-coding RNA (lncRNA) AGAP2-AS1 is an identified oncogene 
      in several types of cancers. However, the role of AGAP2-AS1 in LSCC remains 
      unclear. The expression levels of AGAP2-AS1 in LSCC tissues and cell lines were 
      measured using qRT-PCR. AGAP2-AS1 was knocked down in LSCC cells through 
      transfection with siRNA-AGAP2-AS1. Cell proliferation and invasion were detected 
      using MTT and transwell assays. Dual-luciferase reporter gene assay was performed 
      to confirm the interaction with AGAP2-AS1 and downstream genes. Our results 
      showed that AGAP2-AS1 expression was remarkably increased in human LSCC tissues 
      and cell lines. Knockdown of AGAP2-AS1 significantly inhibited the proliferation 
      and invasion of LSCC cells. In addition, AGAP2-AS1 sponged miR-193a-3p and 
      regulated its expression in LSCC cells. Inhibition of miR-193a-3p reversed the 
      effects of AGAP2-AS1 knockdown on LSCC cells. Furthermore, Lysyl oxidase-like 4 
      (LOXL4) was a target gene of miR-193a-3p and the role of miR-193a-3p was mediated 
      by LOXL4. In conclusion, these findings suggest that knockdown of AGAP2-AS1 
      inhibited the proliferation and invasion of LSCC cells through regulating the 
      miR-193a-3p/LOXL4 axis.
FAU - Ren, Pengyu
AU  - Ren P
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
AD  - Department of Neurosurgery, Second Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
FAU - Niu, Xiaorong
AU  - Niu X
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Zhao, Ruimin
AU  - Zhao R
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Liu, Junsong
AU  - Liu J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Ren, Wanli
AU  - Ren W
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Dai, Hao
AU  - Dai H
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Chen, Jiayu
AU  - Chen J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Yan, Jinfeng
AU  - Yan J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Li, Baiya
AU  - Li B
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Shao, Yuan
AU  - Shao Y
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Bai, Yanxia
AU  - Bai Y
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
FAU - Han, Peng
AU  - Han P
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220203
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 1.4.3.- (LOXL4 protein, human)
RN  - EC 1.4.3.13 (Protein-Lysine 6-Oxidase)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Protein-Lysine 6-Oxidase/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
PMC - PMC8973330
OTO - NOTNLM
OT  - LOXL4
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - invasion
OT  - lncRNA AGAP2-AS1
OT  - miR-193a-3p
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/02/04 06:00
MHDA- 2022/04/08 06:00
PMCR- 2023/02/03
CRDT- 2022/02/03 12:15
PHST- 2022/02/04 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/02/03 12:15 [entrez]
PHST- 2023/02/03 00:00 [pmc-release]
AID - 2016197 [pii]
AID - 10.1080/15384101.2021.2016197 [doi]
PST - ppublish
SO  - Cell Cycle. 2022 Apr;21(7):697-707. doi: 10.1080/15384101.2021.2016197. Epub 2022 
      Feb 3.