PMID- 35114209 OWN - NLM STAT- MEDLINE DCOM- 20220425 LR - 20220425 IS - 1520-6017 (Electronic) IS - 0022-3549 (Linking) VI - 111 IP - 4 DP - 2022 Apr TI - Uptake Kinetics Of Liposomal Formulations of Differing Charge Influences Development of in Vivo Dendritic Cell Immunotherapy. PG - 1081-1091 LID - S0022-3549(22)00032-6 [pii] LID - 10.1016/j.xphs.2022.01.022 [doi] AB - Dendritic cells (DCs) control adaptive immunity and are therefore attractive for in vivo targeting to either induce immune activation or tolerance, depending on disease. Liposomes, nanoparticles comprised of a lipid bi-layer, provide a nanoplatform for loading disease-relevant antigen, adjuvant and DC-targeting molecules simultaneously. However, it is yet not fully understood how liposomal formulations affect uptake by DCs and DC function. Here, we examined monocyte-derived DC (moDC) and skin DC uptake of six different liposomal formulations, together with their DC-modulating effect. Contrary to literature, we show using imaging flow cytometry that anionic or neutral liposomes are taken up more efficiently than cationic liposomes by moDCs, or by skin DCs after intradermal injection. None of the formulations yielded significant modulation of DC function as determined by the upregulation of maturation markers and cytokine production. These results suggest that anionic liposomes would be more suitable as vaccine carriers for a dermal application. CI - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Nagy, Noemi Anna AU - Nagy NA AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. FAU - Castenmiller, Charlotte AU - Castenmiller C AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. FAU - Vigario, Fernando Lozano AU - Vigario FL AD - Division of BioTherapeutics, Leiden Academic Center for Drug Research, Leiden, the Netherlands. FAU - Sparrius, Rinske AU - Sparrius R AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. FAU - van Capel, Toni M M AU - van Capel TMM AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. FAU - de Haas, Aram M AU - de Haas AM AD - Department of Molecular Cell Biology and Immunology, Amsterdam University Medical Center, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. FAU - van Kooyk, Yvette AU - van Kooyk Y AD - Department of Molecular Cell Biology and Immunology, Amsterdam University Medical Center, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. FAU - van Ree, Ronald AU - van Ree R AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands; Department of Otorhinolaryngology, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands. FAU - Tas, Sander W AU - Tas SW AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands; Department of Rheumatology and Clinical Immunology, Amsterdam University Medical Centers, Amsterdam Rheumatology and Immunology Center, University of Amsterdam, Amsterdam, the Netherlands. FAU - Geijtenbeek, Teunis B H AU - Geijtenbeek TBH AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. FAU - Jiskoot, Wim AU - Jiskoot W AD - Division of BioTherapeutics, Leiden Academic Center for Drug Research, Leiden, the Netherlands. FAU - Slutter, Bram AU - Slutter B AD - Division of BioTherapeutics, Leiden Academic Center for Drug Research, Leiden, the Netherlands. FAU - de Jong, Esther C AU - de Jong EC AD - Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: e.c.dejong@amsterdamumc.nl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220131 PL - United States TA - J Pharm Sci JT - Journal of pharmaceutical sciences JID - 2985195R RN - 0 (Immunologic Factors) RN - 0 (Liposomes) SB - IM MH - *Dendritic Cells MH - Immunologic Factors MH - Immunotherapy/methods MH - Kinetics MH - *Liposomes OTO - NOTNLM OT - Cationic lipid(s) OT - Cell culture OT - Drug delivery system(s) OT - Immunogenicity OT - Immunology OT - Immunotherapy OT - Lipid nanoparticle(s) OT - Liposome(s) OT - Nanomedicine OT - Skin COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Payment to institute by Health Holland and Samenwerkende Gezondheidsorganisaties (SGF) Grantnr: LSHM18065-SGF. Payment to institute by Health Holland - TKI-LSH PPP Allowance - Grant nr. LSHM19073, European Commission, NWO-TKI, AB Enzymes, Angany Inc. EDAT- 2022/02/04 06:00 MHDA- 2022/04/26 06:00 CRDT- 2022/02/03 20:12 PHST- 2021/12/29 00:00 [received] PHST- 2022/01/18 00:00 [revised] PHST- 2022/01/18 00:00 [accepted] PHST- 2022/02/04 06:00 [pubmed] PHST- 2022/04/26 06:00 [medline] PHST- 2022/02/03 20:12 [entrez] AID - S0022-3549(22)00032-6 [pii] AID - 10.1016/j.xphs.2022.01.022 [doi] PST - ppublish SO - J Pharm Sci. 2022 Apr;111(4):1081-1091. doi: 10.1016/j.xphs.2022.01.022. Epub 2022 Jan 31.