PMID- 35116405
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220501
IS  - 2219-6803 (Electronic)
IS  - 2218-676X (Print)
IS  - 2218-676X (Linking)
VI  - 10
IP  - 2
DP  - 2021 Feb
TI  - TUG1 promotes retinoblastoma progression by sponging miR-516b-5p to upregulate 
      H6PD expression.
PG  - 738-747
LID - 10.21037/tcr-19-1480 [doi]
AB  - BACKGROUND: Retinoblastoma (RB), depicted as an aggressive eye cancer, mainly 
      occurs in infancy and childhood and is followed by high mortality and poor 
      prognosis. Increasing evidence has revealed that long noncoding RNA taurine 
      upregulated gene 1 (TUG1) is closely linked to the progression of diverse 
      cancers. Nonetheless, the specific function and molecular regulatory mechanism of 
      TUG1 in RB still need to be explored. METHODS: To explore the specific role of 
      TUG1 in RB. TUG1 expression was detected by real-time quantitative polymerase 
      chain reaction (RT-qPCR). Cell counting kit-8 (CCK-8), colony formation, 
      5-ethynyl-2'-deoxyuridine (EdU), caspase-3, terminal-deoxynucleotidyl transferase 
      mediated nick end labeling (TUNEL) and western blot assays were utilized to study 
      the role of TUG1 in RB. The binding relation between miR-516b-5p and TUG1 or 
      hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase (H6PD) was analyzed by 
      luciferase reporter and RNA immunoprecipitation (RIP) assays. RESULTS: The 
      expression of TUG1 was upregulated in RB cells. TUG1 knockdown repressed 
      proliferation ability and promoted apoptosis ability of RB cells. Moreover, TUG1 
      could bind with miR-516b-5p, which targeted H6PD in RB. In addition, the 
      expression of H6PD was negatively and positively regulated by miR-516b-5p and 
      TUG1 in RB, respectively. Finally, H6PD overexpression could partially offset the 
      effects of TUG1 deficiency on cell proliferation and apoptosis. CONCLUSIONS: TUG1 
      promoted the development of RB by sponging miR-516b-5p to upregulate H6PD 
      expression, which might provide a new thought for researching RB-related 
      molecular mechanism.
CI  - 2021 Translational Cancer Research. All rights reserved.
FAU - Xiu, Caimei
AU  - Xiu C
AD  - Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University, Yantai, China.
FAU - Song, Ruiying
AU  - Song R
AD  - Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University, Yantai, China.
FAU - Jiang, Jing
AU  - Jiang J
AD  - Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University, Yantai, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Transl Cancer Res
JT  - Translational cancer research
JID - 101585958
PMC - PMC8799124
OTO - NOTNLM
OT  - Taurine upregulated gene 1 (TUG1)
OT  - hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase (H6PD)
OT  - miR-516b-5p
OT  - retinoblastoma (RB)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at http://dx.doi.org/10.21037/tcr-19-1480). The authors have no 
      conflicts of interest to declare.
EDAT- 2022/02/05 06:00
MHDA- 2022/02/05 06:01
PMCR- 2021/02/01
CRDT- 2022/02/04 05:48
PHST- 2019/08/06 00:00 [received]
PHST- 2020/02/08 00:00 [accepted]
PHST- 2022/02/04 05:48 [entrez]
PHST- 2022/02/05 06:00 [pubmed]
PHST- 2022/02/05 06:01 [medline]
PHST- 2021/02/01 00:00 [pmc-release]
AID - tcr-10-02-738 [pii]
AID - 10.21037/tcr-19-1480 [doi]
PST - ppublish
SO  - Transl Cancer Res. 2021 Feb;10(2):738-747. doi: 10.21037/tcr-19-1480.