PMID- 35126427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220208
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - The LcKNAT1-LcEIL2/3 Regulatory Module Is Involved in Fruitlet Abscission in 
      Litchi.
PG  - 802016
LID - 10.3389/fpls.2021.802016 [doi]
LID - 802016
AB  - Large and premature organ abscission may limit the industrial development of 
      fruit crops by causing serious economic losses. It is well accepted that ethylene 
      (ET) is a strong inducer of organ abscission in plants. However, the mechanisms 
      underlying the control of organ abscission by ET are largely unknown. We 
      previously revealed that LcKNAT1, a KNOTTED-LIKE FROM ARABIDOPSIS THALIANA1 
      (KNAT1)-like protein, acted as a negative regulator in control of fruitlet 
      abscission through suppressing the expression of ET biosynthetic genes in litchi. 
      In this study, we further reported that LcKNAT1 could also directly repress the 
      transcription of LcEIL2 and LcEIL3, two ETHYLENE INSENSITIVE 3-like (EIL) 
      homologs in litchi, which functioned as positive regulators in ET-activated 
      fruitlet abscission by directly promoting the expression of genes responsible for 
      ET biosynthesis and cell wall degradation. The expression level of LcKNAT1 was 
      downregulated, while LcEIL2/3 was upregulated at the abscission zone (AZ) 
      accompanying the fruitlet abscission in litchi. The results of electrophoretic 
      mobility shift assays (EMSAs) and transient expression showed that LcKNAT1 could 
      directly bind to the promoters of LcEIL2 and LcEIL3 and repress their expression. 
      Furthermore, the genetic cross demonstrated that the beta-glucuronidase (GUS) 
      expression driven by the promoters of LcEIL2 or LcEIL3 at the floral AZ was 
      obviously suppressed by LcKNAT1 under stable transformation in Arabidopsis. Taken 
      together, our findings suggest that the LcKNAT1-LcEIL2/3 regulatory module is 
      likely involved in the fruitlet abscission in litchi, and we propose that LcKNAT1 
      could suppress both ET biosynthesis and signaling to regulate litchi fruit 
      abscission.
CI  - Copyright (c) 2022 Ma, Ying, He, Wu, Li and Zhao.
FAU - Ma, Xingshuai
AU  - Ma X
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Ying, Peiyuan
AU  - Ying P
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - He, Zidi
AU  - He Z
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Wu, Hong
AU  - Wu H
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Li, Jianguo
AU  - Li J
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Zhao, Minglei
AU  - Zhao M
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, Key Laboratory of Biology and Germplasm Enhancement of 
      Horticultural Crops in South China at Ministry of Agriculture and Rural Affair, 
      Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and 
      Vegetables, College of Horticulture, South China Agricultural University, 
      Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220121
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8813966
OTO - NOTNLM
OT  - LcEIL2/3
OT  - LcKNAT1
OT  - ethylene
OT  - fruitlet abscission
OT  - litchi (Litchi chinensis Sonn.)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/02/08 06:00
MHDA- 2022/02/08 06:01
PMCR- 2021/01/01
CRDT- 2022/02/07 05:30
PHST- 2021/10/26 00:00 [received]
PHST- 2021/12/15 00:00 [accepted]
PHST- 2022/02/07 05:30 [entrez]
PHST- 2022/02/08 06:00 [pubmed]
PHST- 2022/02/08 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.802016 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Jan 21;12:802016. doi: 10.3389/fpls.2021.802016. 
      eCollection 2021.