PMID- 35131263
OWN - NLM
STAT- MEDLINE
DCOM- 20220415
LR  - 20220531
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 298
IP  - 3
DP  - 2022 Mar
TI  - RNA-dependent synthesis of ergosteryl-3beta-O-glycine in Ascomycota expands the 
      diversity of steryl-amino acids.
PG  - 101657
LID - S0021-9258(22)00097-7 [pii]
LID - 10.1016/j.jbc.2022.101657 [doi]
LID - 101657
AB  - A wide range of bacteria possess virulence factors such as aminoacyl-tRNA 
      transferases (ATTs) that are capable of rerouting aminoacyl-transfer RNAs away 
      from protein synthesis to conjugate amino acids onto glycerolipids. We recently 
      showed that, although these pathways were thought to be restricted to bacteria, 
      higher fungi also possess ergosteryl-3beta-O-L-aspartate synthases (ErdSs), which 
      transfer the L-Asp moiety of aspartyl-tRNA(Asp) onto the 3beta-OH group of 
      ergosterol (Erg), yielding ergosteryl-3beta-O-L-aspartate (Erg-Asp). Here, we report 
      the discovery, in fungi, of a second type of fungal sterol-specific ATTs, namely, 
      ergosteryl-3beta-O-glycine (Erg-Gly) synthase (ErgS). ErgS consists of a 
      freestanding DUF2156 domain encoded by a gene distinct from and paralogous to 
      that of ErdS. We show that the enzyme only uses Gly-tRNA(Gly) produced by an 
      independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of 
      Erg, producing Erg-Gly. Phylogenomics analysis also show that the Erg-Gly 
      synthesis pathway exists only in Ascomycota, including species of 
      biotechnological interest, and more importantly, in human pathogens, such as 
      Aspergillus fumigatus. The discovery of a second type of Erg-aa not only expands 
      the repertoire of this particular class of fungal lipids but suggests that Erg-aa 
      synthases might constitute a genuine subfamily of lipid-modifying ATTs.
CI  - Published by Elsevier Inc.
FAU - Yakobov, Nathaniel
AU  - Yakobov N
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France.
FAU - Mahmoudi, Nassira
AU  - Mahmoudi N
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France. Electronic address: 
      nmahmoudikaidi@unistra.fr.
FAU - Grob, Guillaume
AU  - Grob G
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France.
FAU - Yokokawa, Daisuke
AU  - Yokokawa D
AD  - School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
FAU - Saga, Yusuke
AU  - Saga Y
AD  - School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
FAU - Kushiro, Tetsuo
AU  - Kushiro T
AD  - School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
FAU - Worrell, Danielle
AU  - Worrell D
AD  - Burnett School of Biomedical Sciences, College of Medicine, University of Central 
      Florida, Orlando, Florida, USA.
FAU - Roy, Herve
AU  - Roy H
AD  - Burnett School of Biomedical Sciences, College of Medicine, University of Central 
      Florida, Orlando, Florida, USA.
FAU - Schaller, Hubert
AU  - Schaller H
AD  - Plant Isoprenoid Biology (PIB) Team, Institut de Biologie Moleculaire des Plantes 
      du CNRS, Universite de Strasbourg, Strasbourg, France.
FAU - Senger, Bruno
AU  - Senger B
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France.
FAU - Huck, Laurence
AU  - Huck L
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France.
FAU - Riera Gascon, Gisela
AU  - Riera Gascon G
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France.
FAU - Becker, Hubert D
AU  - Becker HD
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France. Electronic address: h.becker@unistra.fr.
FAU - Fischer, Frederic
AU  - Fischer F
AD  - CNRS, Genetique Moleculaire, Genomique, Microbiologie, UMR 7156, Universite de 
      Strasbourg, Strasbourg Cedex, France. Electronic address: frfischer@unistra.fr.
LA  - eng
GR  - R21 AI144481/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20220204
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Acids)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Transfer, Amino Acyl)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - TE7660XO1C (Glycine)
RN  - Z30RAY509F (Ergosterol)
SB  - IM
MH  - Amino Acids
MH  - *Ascomycota/genetics/metabolism
MH  - Aspartic Acid
MH  - *Ergosterol
MH  - *Glycine/biosynthesis/genetics/metabolism
MH  - Humans
MH  - RNA, Fungal/genetics/metabolism
MH  - RNA, Transfer, Amino Acyl/genetics/metabolism
PMC - PMC8913301
OTO - NOTNLM
OT  - DUF2156
OT  - aminoacyl-tRNA
OT  - ergosterol
OT  - ergosteryl-3beta-O-L-aspartate
OT  - ergosteryl-3beta-O-glycine
OT  - fungi
OT  - sterol aminoacylation
COIS- Conflict of interest The authors declare that they have no conflicts of interest 
      with the contents of this article.
EDAT- 2022/02/09 06:00
MHDA- 2022/04/16 06:00
PMCR- 2022/02/04
CRDT- 2022/02/08 05:40
PHST- 2021/07/27 00:00 [received]
PHST- 2022/01/13 00:00 [revised]
PHST- 2022/01/16 00:00 [accepted]
PHST- 2022/02/09 06:00 [pubmed]
PHST- 2022/04/16 06:00 [medline]
PHST- 2022/02/08 05:40 [entrez]
PHST- 2022/02/04 00:00 [pmc-release]
AID - S0021-9258(22)00097-7 [pii]
AID - 101657 [pii]
AID - 10.1016/j.jbc.2022.101657 [doi]
PST - ppublish
SO  - J Biol Chem. 2022 Mar;298(3):101657. doi: 10.1016/j.jbc.2022.101657. Epub 2022 
      Feb 4.