PMID- 35132758
OWN - NLM
STAT- MEDLINE
DCOM- 20220421
LR  - 20220722
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Linking)
VI  - 23
IP  - 8
DP  - 2022 Apr 20
TI  - A Four-Step Enzymatic Cascade for Efficient Production of L-Phenylglycine from 
      Biobased L-Phenylalanine.
PG  - e202100661
LID - 10.1002/cbic.202100661 [doi]
AB  - Enantiopure amino acids are of particular interest in the agrochemical and 
      pharmaceutical industries. Here, we report a multi-enzyme cascade for efficient 
      production of L-phenylglycine (L-Phg) from biobased L-phenylalanine (L-Phe). We 
      first attempted to engineer Escherichia coli for expressing L-amino acid 
      deaminase (LAAD) from Proteus mirabilis, hydroxymandelate synthase (HmaS) from 
      Amycolatopsis orientalis, (S)-mandelate dehydrogenase (SMDH) from Pseudomonas 
      putida, the endogenous aminotransferase (AT) encoded by ilvE and L-glutamate 
      dehydrogenase (GluDH) from E. coli. However, 10 mM L-Phe only afforded the 
      synthesis of 7.21+/-0.15 mM L-Phg. The accumulation of benzoylformic acid suggested 
      that the transamination step might be rate-limiting. We next used leucine 
      dehydrogenase (LeuDH) from Bacillus cereus to bypass the use of L-glutamate as 
      amine donor, and 40 mM L-Phe gave 39.97+/-3.84 mM (6.04+/-0.58 g/L) L-Phg, reaching 
      99.9 % conversion. In summary, this work demonstrates a concise four-step 
      enzymatic cascade for L-Phg synthesis from biobased L-Phe, with a potential for 
      future industrial applications.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Zhu, Yuling
AU  - Zhu Y
AD  - State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen 
      University, Fujian, 361102, China.
FAU - Yuan, Jifeng
AU  - Yuan J
AUID- ORCID: 0000-0003-1874-190X
AD  - State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen 
      University, Fujian, 361102, China.
LA  - eng
GR  - 0660-X2123310/Xiamen University/
GR  - ZhenSheng Biotech, China/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220226
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Amino Acids)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 69-91-0 (2-phenylglycine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Amino Acids/metabolism
MH  - *Escherichia coli/metabolism
MH  - Glycine/analogs & derivatives/metabolism
MH  - *Phenylalanine/metabolism
OTO - NOTNLM
OT  - L-amino acid deaminase
OT  - L-phenylglycine
OT  - leucine dehydrogenases
OT  - multi-enzyme cascade
OT  - whole-cell biocatalyst
EDAT- 2022/02/09 06:00
MHDA- 2022/04/22 06:00
CRDT- 2022/02/08 05:54
PHST- 2022/02/06 00:00 [revised]
PHST- 2021/12/02 00:00 [received]
PHST- 2022/02/09 06:00 [pubmed]
PHST- 2022/04/22 06:00 [medline]
PHST- 2022/02/08 05:54 [entrez]
AID - 10.1002/cbic.202100661 [doi]
PST - ppublish
SO  - Chembiochem. 2022 Apr 20;23(8):e202100661. doi: 10.1002/cbic.202100661. Epub 2022 
      Feb 26.