PMID- 35133290
OWN - NLM
STAT- MEDLINE
DCOM- 20220325
LR  - 20220531
IS  - 1741-7899 (Electronic)
IS  - 1470-1626 (Linking)
VI  - 163
IP  - 4
DP  - 2022 Feb 24
TI  - Regulation of Tjp1 mRNA by CPEB2 for tight junction assembly in mouse blastocyst.
PG  - 233-240
LID - 10.1530/REP-21-0227 [doi]
AB  - Cytoplasmic polyadenylation element-binding protein 2 (CPEB2) is an mRNA-binding 
      protein that regulates the cytoplasmic polyadenylation of mRNA and is required 
      for tight junction (TJ) assembly in the trophectoderm epithelium during porcine 
      preimplantation development. However, the regulatory mechanism underlying TJ 
      assembly by CPEB2 has not been examined. The aim of this study was to elucidate 
      how Cpeb2 regulates the subcellular localisation and stabilisation of Tjp1 mRNA 
      for TJ biogenesis during mouse preimplantation. CPEB2 was detected in nuclei 
      during the early stages of development and was localised at apical cell membranes 
      from the morula stage onwards. In the Cpeb2 knockdown (KD), we observed reduced 
      blastocyst formation with impaired TJs, defective inner cell mass development in 
      the blastocyst outgrowth assay, and loss of pregnancy after embryo transfer. More 
      importantly, Tjp1 mRNA was localised apically in the outer cells of control 
      morulae but not in the Cpeb2 KD embryos, indicating that CPEB2 mediated the 
      translocalisation of Tjp1 mRNA from the nuclei. Finally, in the control embryos, 
      the length of the Tjp1 mRNA poly (A) tail was varied, while only a single peak 
      was detected in the Cpeb2 KD embryos. These findings suggest that the binding of 
      CPEB2 to the cytoplasmic polyadenylation element in the 3'-UTR can confer 
      stability on Tjp1 mRNA and translational regulation. In summary, we demonstrated 
      for the first time that CPEB2 mediates Tjp1 mRNA stabilisation and subcellular 
      localisation for TJ assembly during mouse blastocyst formation.
FAU - Jeong, Yelin
AU  - Jeong Y
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Korea, Republic of Korea.
FAU - Lee, Seulah
AU  - Lee S
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Korea, Republic of Korea.
FAU - Choi, Inchul
AU  - Choi I
AUID- ORCID: 0000-0001-5011-2658
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Korea, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220224
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (CPEB2 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Zonula Occludens-1 Protein)
SB  - IM
MH  - Animals
MH  - *Blastocyst
MH  - Embryonic Development
MH  - Mice
MH  - Morula/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Swine
MH  - *Tight Junctions/metabolism
MH  - Zonula Occludens-1 Protein/metabolism
EDAT- 2022/02/09 06:00
MHDA- 2022/03/26 06:00
CRDT- 2022/02/08 12:14
PHST- 2021/05/27 00:00 [received]
PHST- 2022/02/08 00:00 [accepted]
PHST- 2022/02/09 06:00 [pubmed]
PHST- 2022/03/26 06:00 [medline]
PHST- 2022/02/08 12:14 [entrez]
AID - 10.1530/REP-21-0227 [doi]
PST - epublish
SO  - Reproduction. 2022 Feb 24;163(4):233-240. doi: 10.1530/REP-21-0227.