PMID- 35142063
OWN - NLM
STAT- MEDLINE
DCOM- 20221031
LR  - 20221101
IS  - 1742-481X (Electronic)
IS  - 1742-4801 (Print)
IS  - 1742-4801 (Linking)
VI  - 19
IP  - 7
DP  - 2022 Nov
TI  - In situ cell electrospun using a portable handheld electrospinning apparatus for 
      the repair of wound healing in rats.
PG  - 1693-1704
LID - 10.1111/iwj.13769 [doi]
AB  - Slow or non-healing wounds caused by full-thickness skin wounds of various 
      origins have become a difficult challenge in clinical wound treatment. In 
      particular, large full-thickness skin wounds often lead to serious chronic skin 
      wounds that do not heal. Electrospinning technology and stem cell treatment for 
      wound repair have attracted much attention due to its unique advantages. In the 
      current study， we electrospun polyvinyl alcohol (PVA) and bone marrow-derived 
      stem cells (BMSCs) by a handheld electrospinning device, the distribution and 
      interaction of cells and fibres were determined by light and electron microscopy 
      and the cell viability and proliferation were determined by live/dead cell 
      staining. The tissues were analysed by histology with Haematoxylin and Eosin 
      (H&E) and Masson staining and immunohistochemical staining. We found that the 
      fibres were distributed uniformly and BMSCs were distributed between the fibres. 
      Cytotoxicity and cell proliferation tests proved its good biocompatibility. 
      Histological staining shows it can accelerate wound healing and appendages 
      regeneration by promoting granulation tissue repair. The instant PVA/stem cell 
      fibres prepared by a handheld electrospinning device strongly promote the repair 
      of full-thickness skin wounds in rats. The proposed electrospinning technology is 
      expected to have great potential in household, outdoor and battlefield first aid.
CI  - (c) 2022 The Authors. International Wound Journal published by Medicalhelplines.com 
      Inc (3M) and John Wiley & Sons Ltd.
FAU - Xu, Shunen
AU  - Xu S
AD  - Medical College of Soochow University, Suzhou, China.
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
FAU - Lu, Tao
AU  - Lu T
AD  - Department of Orthopaedics, The First People's Hospital of Guiyang, Guiyang, 
      China.
FAU - Yang, Long
AU  - Yang L
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
FAU - Luo, Siwei
AU  - Luo S
AD  - Guizhou Medical University, Guiyang, China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Guizhou Medical University, Guiyang, China.
FAU - Ye, Chuan
AU  - Ye C
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
AD  - Key Laboratory of Adult Stem Cell Transformation Research, Chinese Academy of 
      Medical Sciences, Guiyang, China.
AD  - Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, 
      Guiyang, China.
LA  - eng
GR  - gzwkj2021-233/Science and Technology Fund Project of Guizhou Health Commission/
PT  - Journal Article
DEP - 20220210
PL  - England
TA  - Int Wound J
JT  - International wound journal
JID - 101230907
RN  - 9002-89-5 (Polyvinyl Alcohol)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Wound Healing
MH  - *Polyvinyl Alcohol/therapeutic use
MH  - Stem Cells
PMC - PMC9615271
OTO - NOTNLM
OT  - BMSCs
OT  - PVA
OT  - cell electrospun
OT  - tissue engineering
OT  - wound
COIS- The authors declare no conflicts of interest.
EDAT- 2022/02/11 06:00
MHDA- 2022/11/01 06:00
PMCR- 2022/02/10
CRDT- 2022/02/10 05:44
PHST- 2022/01/20 00:00 [revised]
PHST- 2021/11/05 00:00 [received]
PHST- 2022/01/24 00:00 [accepted]
PHST- 2022/02/11 06:00 [pubmed]
PHST- 2022/11/01 06:00 [medline]
PHST- 2022/02/10 05:44 [entrez]
PHST- 2022/02/10 00:00 [pmc-release]
AID - IWJ13769 [pii]
AID - 10.1111/iwj.13769 [doi]
PST - ppublish
SO  - Int Wound J. 2022 Nov;19(7):1693-1704. doi: 10.1111/iwj.13769. Epub 2022 Feb 10.