PMID- 35142935
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220520
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 53
IP  - 2
DP  - 2022 Apr
TI  - Circ_0120175 promotes laryngeal squamous cell carcinoma development through 
      up-regulating SLC7A11 by sponging miR-330-3p.
PG  - 159-171
LID - 10.1007/s10735-022-10061-1 [doi]
AB  - The aim of our study was to illustrate the role of circular RNA 0120175 
      (circ_0120175) and its associated mechanism in laryngeal squamous cell carcinoma 
      (LSCC) development. The abundance of circ_0120175, microRNA-330-3p (miR-330-3p) 
      and solute carrier family 7, membrane 11 (SLC7A11) messenger RNA and protein was 
      measured by quantitative real time polymerase chain reaction and Western blot 
      assay. Cell proliferation, apoptosis, migration and invasion were assessed by 
      cell counting kit-8 assay, flow cytometry and transwell migration and invasion 
      assays, respectively. The interaction between miR-330-3p and circ_0120175 or 
      SLC7A11 was confirmed by dual-luciferase reporter assay. Murine xenograft model 
      was established to test the function of circ_0120175 in tumor growth in vivo. 
      Circ_0120175 abundance was aberrantly increased in LSCC tissues and cell lines, 
      and LSCC patients with high level of circ_0120175 were associated with advanced 
      tumor staging, lymph node metastasis and short survival time. Circ_0120175 
      interference suppressed cell proliferation, migration and invasion and induced 
      cell apoptosis of LSCC cells. Circ_0120175 could sponge and negatively regulate 
      miR-330-3p expression in LSCC cells. The addition of anti-miR-330-3p partly 
      reversed circ_0120175 knockdown-induced effects in LSCC cells. SLC7A11 bound to 
      miR-330-3p. Circ_0120175 enhanced the abundance of SLC7A11 through sponging 
      miR-330-3p in LSCC cells. Circ_0120175 silencing-mediated influences in LSCC 
      cells were partly counteracted by the overexpression of SLC7A11. Circ_0120175 
      interference notably suppressed xenograft tumor growth in vivo. Circ_0120175 
      promoted proliferation, migration and invasion while impeded cell apoptosis of 
      LSCC cells through miR-330-3p/SLC7A11 axis, which provided novel therapeutic 
      targets for LSCC.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Fan, Daqing
AU  - Fan D
AD  - Department of Otolaryngology-Head and Neck Surgery, Hubei Cancer Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, No. 116 South 
      Zhuodaoquan Road, Hongshan District, Wuhan, 430079, Hubei, China. zatvma@163.com.
FAU - Zhu, Youhua
AU  - Zhu Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Hubei Cancer Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, No. 116 South 
      Zhuodaoquan Road, Hongshan District, Wuhan, 430079, Hubei, China.
LA  - eng
PT  - Journal Article
DEP - 20220210
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Amino Acid Transport System y+)
RN  - 0 (MIRN330 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (SLC7A11 protein, human)
SB  - IM
MH  - Amino Acid Transport System y+
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Mice
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
OTO - NOTNLM
OT  - LSCC
OT  - SLC7A11
OT  - circ_0120175
OT  - miR-330-3p
EDAT- 2022/02/11 06:00
MHDA- 2022/05/21 06:00
CRDT- 2022/02/10 12:15
PHST- 2020/10/26 00:00 [received]
PHST- 2022/01/26 00:00 [accepted]
PHST- 2022/02/11 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/02/10 12:15 [entrez]
AID - 10.1007/s10735-022-10061-1 [pii]
AID - 10.1007/s10735-022-10061-1 [doi]
PST - ppublish
SO  - J Mol Histol. 2022 Apr;53(2):159-171. doi: 10.1007/s10735-022-10061-1. Epub 2022 
      Feb 10.