PMID- 35143519
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 2
DP  - 2022
TI  - LysM receptors in Coffea arabica: Identification, characterization, and gene 
      expression in response to Hemileia vastatrix.
PG  - e0258838
LID - 10.1371/journal.pone.0258838 [doi]
LID - e0258838
AB  - Pathogen-associated molecular patterns (PAMPs) are recognized by pattern 
      recognition receptors (PRRs) localized on the host plasma membrane. These 
      receptors activate a broad-spectrum and durable defense, which are desired 
      characteristics for disease resistance in plant breeding programs. In this study, 
      candidate sequences for PRRs with lysin motifs (LysM) were investigated in the 
      Coffea arabica genome. For this, approaches based on the principle of sequence 
      similarity, conservation of motifs and domains, phylogenetic analysis, and 
      modulation of gene expression in response to Hemileia vastatrix were used. The 
      candidate sequences for PRRs in C. arabica (Ca1-LYP, Ca2-LYP, Ca1-CERK1, 
      Ca2-CERK1, Ca-LYK4, Ca1-LYK5 and Ca2-LYK5) showed high similarity with the 
      reference PRRs used: Os-CEBiP, At-CERK1, At-LYK4 and At-LYK5. Moreover, the 
      ectodomains of these sequences showed high identity or similarity with the 
      reference sequences, indicating structural and functional conservation. The 
      studied sequences are also phylogenetically related to the reference PRRs 
      described in Arabidopsis, rice, and other plant species. All candidates for 
      receptors had their expression induced after the inoculation with H. vastatrix, 
      since the first time of sampling at 6 hours post-inoculation (hpi). At 24 hpi, 
      there was a significant increase in expression, for most of the receptors 
      evaluated, and at 48 hpi, a suppression. The results showed that the candidate 
      sequences for PRRs in the C. arabica genome display high homology with fungal 
      PRRs already described in the literature. Besides, they respond to pathogen 
      inoculation and seem to be involved in the perception or signaling of fungal 
      chitin, acting as receptors or co-receptors of this molecule. These findings 
      represent an advance in the understanding of the basal immunity of this species.
FAU - Santos, Mariana de Lima
AU  - Santos ML
AUID- ORCID: 0000-0003-0642-2696
AD  - Programa de Pos-graduacao em Biotecnologia Vegetal, Universidade Federal de 
      Lavras, Lavras, Minas Gerais, Brazil.
FAU - de Resende, Mario Lucio Vilela
AU  - de Resende MLV
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
FAU - Dos Santos Ciscon, Barbara Alves
AU  - Dos Santos Ciscon BA
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
FAU - Freitas, Natalia Chagas
AU  - Freitas NC
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
FAU - Pereira, Matheus Henrique de Brito
AU  - Pereira MHB
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
FAU - Reichel, Tharyn
AU  - Reichel T
AUID- ORCID: 0000-0003-2754-0245
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
FAU - Mathioni, Sandra Marisa
AU  - Mathioni SM
AD  - Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas 
      Gerais, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220210
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Receptors, Pattern Recognition)
RN  - Hemileia vastatrix
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/genetics
MH  - Arabidopsis Proteins/genetics
MH  - Basidiomycota/*genetics/physiology
MH  - Coffea/*genetics/metabolism/microbiology
MH  - Fungal Proteins/genetics/metabolism
MH  - Gene Expression
MH  - Genome, Plant
MH  - Oryza/genetics
MH  - Phylogeny
MH  - Plant Diseases/microbiology
MH  - Plant Proteins/classification/*genetics/metabolism
MH  - Receptors, Pattern Recognition/classification/*genetics/metabolism
MH  - Sequence Alignment
PMC - PMC8830669
COIS- The authors have declared that no competing interests exist. Therefore, this does 
      not alter our adherence to PLOS ONE policies on sharing data and materials.
EDAT- 2022/02/11 06:00
MHDA- 2022/02/26 06:00
PMCR- 2022/02/10
CRDT- 2022/02/10 17:15
PHST- 2021/10/05 00:00 [received]
PHST- 2022/01/28 00:00 [accepted]
PHST- 2022/02/10 17:15 [entrez]
PHST- 2022/02/11 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2022/02/10 00:00 [pmc-release]
AID - PONE-D-21-32076 [pii]
AID - 10.1371/journal.pone.0258838 [doi]
PST - epublish
SO  - PLoS One. 2022 Feb 10;17(2):e0258838. doi: 10.1371/journal.pone.0258838. 
      eCollection 2022.