PMID- 35145404
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220216
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 12
DP  - 2021
TI  - Practical Protocol for Comprehensively Evaluating Sulfur-Fumigation of Baizhi 
      Based on Metabolomics, Pharmacology, and Cytotoxicity.
PG  - 799504
LID - 10.3389/fphar.2021.799504 [doi]
LID - 799504
AB  - Sulfur Angelicae Dahuricae Radix (Baizhi) is a common medicinal herb in Asian 
      countries. A practical protocol combining metabolomics, pharmacology, and 
      cytotoxicity was developed to comprehensively evaluate the influence of 
      sulfur-fumigation on the quality of Baizhi. Furocoumarins could be transformed 
      into sulfur-containing compounds during the sulfuring process, among which 1 and 
      3 were purified with relatively high abundance and identified as 
      3,4-dihydrobyakangelicin-4-sulfonic acid and (4R,12S)-3,4-dihydrooxypeucedanin 
      hydrate-4-sulfonic acid (OXH-S), respectively. OXH-S was found to be an addition 
      product of sulfite and oxypeucedanin hydrate (OXH-N). Then, the cytotoxicity and 
      anti-inflammatory activity of OXH-N, OXH-S, and water extracts of sulfured 
      (extraction-S), and unsulfured Baizhi (extraction-N) were evaluated. OXH-S and 
      extraction-S were less toxic than OXH-N and extraction-N, respectively. A 
      comparison of OXH-N with OXH-S and extraction-N with extraction-S showed no 
      significant differences in anti-inflammatory activity. These results suggest that 
      sulfur fumigation can reduce toxicity and does not influence the 
      anti-inflammatory activity of Baizhi, even after chemical composition changes. 
      The proposed protocol based on marker screening, pharmacology, and safety 
      evaluation provides a scientific basis for the standardization and regulation of 
      sulfured Baizhi and other medical materials.
CI  - Copyright (c) 2022 Deng, Kang, Kang, Lyu, Zhang, Wang, Wang, Nan, Zhou, Huang, Zhan 
      and Guo.
FAU - Deng, Ai-Ping
AU  - Deng AP
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Kang, Chuan-Zhi
AU  - Kang CZ
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Kang, Li-Ping
AU  - Kang LP
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Lyu, Chao-Geng
AU  - Lyu CG
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Zhang, Wen-Jin
AU  - Zhang WJ
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Wang, Sheng
AU  - Wang S
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Wang, Hong-Yang
AU  - Wang HY
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Nan, Tie-Gui
AU  - Nan TG
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Zhou, Li
AU  - Zhou L
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Huang, Lu-Qi
AU  - Huang LQ
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Zhan, Zhi-Lai
AU  - Zhan ZL
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
FAU - Guo, Lan-Ping
AU  - Guo LP
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for 
      Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220125
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC8822044
OTO - NOTNLM
OT  - Angelica dahurica
OT  - cytotoxicity
OT  - furocoumarin
OT  - inflammatory
OT  - marker
OT  - sulfur-fumigation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/02/12 06:00
MHDA- 2022/02/12 06:01
PMCR- 2022/01/25
CRDT- 2022/02/11 05:38
PHST- 2021/10/30 00:00 [received]
PHST- 2021/12/29 00:00 [accepted]
PHST- 2022/02/11 05:38 [entrez]
PHST- 2022/02/12 06:00 [pubmed]
PHST- 2022/02/12 06:01 [medline]
PHST- 2022/01/25 00:00 [pmc-release]
AID - 799504 [pii]
AID - 10.3389/fphar.2021.799504 [doi]
PST - epublish
SO  - Front Pharmacol. 2022 Jan 25;12:799504. doi: 10.3389/fphar.2021.799504. 
      eCollection 2021.