PMID- 35146836
OWN - NLM
STAT- MEDLINE
DCOM- 20221102
LR  - 20221229
IS  - 1549-8719 (Electronic)
IS  - 1073-9688 (Linking)
VI  - 29
IP  - 6-7
DP  - 2022 Oct
TI  - Optical method to determine in vivo capillary hematocrit, hemoglobin 
      concentration, and 3-D network geometry in skeletal muscle.
PG  - e12751
LID - 10.1111/micc.12751 [doi]
AB  - OBJECTIVE: The aim of this study was to develop a tool to visualize and quantify 
      hemodynamic information, such as hemoglobin concentration and hematocrit, within 
      microvascular networks recorded in vivo using intravital video microscopy. 
      Additionally, we aimed to facilitate the 3-D reconstruction of the microvascular 
      networks. METHODS: Digital images taken from an intravital video microscopy 
      preparation of the extensor digitorum longus muscle in rats for 25 capillary 
      segments were used. The developed algorithm was used to delineate capillaries of 
      interest, calculate the optical density for each pixel in the image, and 
      reconstruct the 3-D capillary geometry using the calculated light path-lengths. 
      Subsequently, the mean corpuscular hemoglobin concentration (MCHC), hemoglobin 
      concentration, and hematocrit for these capillaries were calculated. We evaluated 
      the hematocrit values determined by our methodology by comparing them to those 
      obtained using a previously published method. RESULTS: The hematocrit values from 
      the proposed optical method were strongly correlated with those calculated using 
      published methods r(2) (25) = .92, p < .001, and demonstrated excellent agreement 
      with a mean difference of 1.3% and a coefficient of variation (CV) of 11%. The 
      average MCHC, hemoglobin concentration, and light path-lengths were 23.83 g/dl, 
      8.06 g/dl, and 3.92 microm, respectively. CONCLUSION: The proposed methodology can 
      quantify hemodynamic measurements and produce functional images for visualization 
      of the microcirculation in vivo.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Christie, Jaryd R
AU  - Christie JR
AUID- ORCID: 0000-0001-7063-0401
AD  - Department of Medical Biophysics, University of Western Ontario, London, Ontario, 
      Canada.
FAU - Kong, Isaac
AU  - Kong I
AUID- ORCID: 0000-0002-5765-1715
AD  - Division of Radiation Oncology, Department of Oncology, Juravinski Cancer Centre, 
      McMaster University, Hamilton, Ontario, Canada.
FAU - Mawdsley, Laura
AU  - Mawdsley L
AD  - Department of Medical Biophysics, University of Western Ontario, London, Ontario, 
      Canada.
AD  - Robarts Research Institute, University of Western Ontario, London, Ontario, 
      Canada.
FAU - Milkovich, Stephanie
AU  - Milkovich S
AD  - Department of Medical Biophysics, University of Western Ontario, London, Ontario, 
      Canada.
AD  - Robarts Research Institute, University of Western Ontario, London, Ontario, 
      Canada.
FAU - Doornekamp, Ande
AU  - Doornekamp A
AD  - Department of Medical Biophysics, University of Western Ontario, London, Ontario, 
      Canada.
FAU - Baek, Jason
AU  - Baek J
AD  - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
FAU - Fraser, Graham M
AU  - Fraser GM
AUID- ORCID: 0000-0002-5733-201X
AD  - Division of BioMedical Sciences, Memorial University of Newfoundland, St. John's, 
      NL, Canada.
FAU - Ellis, Christopher G
AU  - Ellis CG
AUID- ORCID: 0000-0002-7760-1846
AD  - Department of Medical Biophysics, University of Western Ontario, London, Ontario, 
      Canada.
AD  - Robarts Research Institute, University of Western Ontario, London, Ontario, 
      Canada.
FAU - Sove, Richard J
AU  - Sove RJ
AD  - Department of Biomedical Engineering, Johns Hopkins University, Baltimore, 
      Maryland, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220222
PL  - United States
TA  - Microcirculation
JT  - Microcirculation (New York, N.Y. : 1994)
JID - 9434935
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Capillaries/diagnostic imaging/physiology
MH  - Hematocrit
MH  - *Muscle, Skeletal/diagnostic imaging/blood supply
MH  - Microcirculation/physiology
MH  - Hemoglobins
OTO - NOTNLM
OT  - hematocrit
OT  - hemoglobin
OT  - in vivo
OT  - microvascular network geometry
OT  - optical density
EDAT- 2022/02/12 06:00
MHDA- 2022/11/03 06:00
CRDT- 2022/02/11 05:47
PHST- 2021/12/18 00:00 [revised]
PHST- 2021/07/31 00:00 [received]
PHST- 2022/02/02 00:00 [accepted]
PHST- 2022/02/12 06:00 [pubmed]
PHST- 2022/11/03 06:00 [medline]
PHST- 2022/02/11 05:47 [entrez]
AID - 10.1111/micc.12751 [doi]
PST - ppublish
SO  - Microcirculation. 2022 Oct;29(6-7):e12751. doi: 10.1111/micc.12751. Epub 2022 Feb 
      22.