PMID- 35148960
OWN - NLM
STAT- MEDLINE
DCOM- 20220420
LR  - 20221108
IS  - 2213-2945 (Print)
IS  - 2213-2953 (Linking)
VI  - 11
IP  - 3
DP  - 2022 May-Jun
TI  - Multiplex fluorescence in situ hybridization testing for anaplastic lymphoma 
      kinase and c-ros oncogene 1 gene rearrangements on cytology smears in lung 
      adenocarcinomas: comparative study with formalin-fixed paraffin-embedded 
      sections.
PG  - 154-164
LID - S2213-2945(22)00001-1 [pii]
LID - 10.1016/j.jasc.2022.01.001 [doi]
AB  - INTRODUCTION: Multiplex anaplastic lymphoma kinase (ALK)/c-ros oncogene 1 (ROS1) 
      fluorescence in situ hybridization (FISH) probes conserve tissue by analyzing 
      both ALK and ROS1 gene rearrangements (ALK-R/ROS1-R) in a single test. The 
      positivity cutoffs have been validated on formalin-fixed, paraffin-embedded 
      (FFPE) tissue sections and not tested on non-cell block (CB) cytology 
      preparations. We sought to validate non-CB cytology preparations for the 
      detection of ALK-R/ROS1-R using multiplex ALK/ROS1 FISH probes by comparing the 
      results with matched FFPE results. MATERIALS AND METHODS: During the 3.5-year 
      study period, FISH using the FlexISH ALK/ROS1 DistinguISH Probe (ZytoVision) was 
      performed in non-CB cytology preparations of patients for whom FISH on FFPE 
      sections was performed. RESULTS: A total of 20 patients had one or more non-CB 
      cytology preparations (n = 27) suitable for FISH analysis. These comprised direct 
      smears (n = 17), smears from centrifuged effusion pellets (n = 8), cytospin 
      smears (n = 1), and biopsy imprint smears (n = 1). These had been fixed in 95% 
      ethanol (n = 18) or air dried (n = 9), and stained with Papanicolaou (n = 14), 
      May-Grunwald-Giemsa (n = 9), immunocytochemistry (n = 3), or hematoxylin and 
      eosin (n = 1). The median archival time was 1 year. Successful FISH results were 
      achieved in 14 samples (6 with ALK-R, 2 with ROS1-R, 6 negative) and were 
      concordant with the FFPE FISH results for 13 of 14 cases. The single case with 
      discordant results between cytology and FFPE FISH showed ALK-R on cytology 
      concordant with positive ALK D5F3 companion diagnostics assay results and was 
      considered a false-negative FFPE FISH result. FISH failure occurred mainly in the 
      older archived slides because of overdigestion (n = 5), hybridization failure (n 
      = 5), or excessive background fluorescence (n = 3). CONCLUSIONS: Non-CB cytology 
      smears are highly suitable for multiplex FISH analysis with 100% concordance with 
      FFPE FISH and/or ALK D5F3 companion diagnostics assay results.
CI  - Copyright (c) 2022 American Society of Cytopathology. Published by Elsevier Inc. 
      All rights reserved.
FAU - Nambirajan, Aruna
AU  - Nambirajan A
AD  - Departments of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Rana, Deeksha
AU  - Rana D
AD  - Departments of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Samant, Komal
AU  - Samant K
AD  - Departments of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Prabakaran, Aswini
AU  - Prabakaran A
AD  - Departments of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Malik, Prabhat
AU  - Malik P
AD  - Department of Medical Oncology, Dr. B.R.A.IRCH, All India Institute of Medical 
      Sciences, New Delhi, India.
FAU - Jain, Deepali
AU  - Jain D
AD  - Departments of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India. Electronic address: deepalijain76@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220113
PL  - United States
TA  - J Am Soc Cytopathol
JT  - Journal of the American Society of Cytopathology
JID - 101613234
RN  - 0 (Proto-Oncogene Proteins)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - *Adenocarcinoma of Lung
MH  - Anaplastic Lymphoma Kinase/genetics
MH  - Formaldehyde
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Lung Neoplasms/pathology
MH  - Oncogenes
MH  - Paraffin Embedding
MH  - Protein-Tyrosine Kinases/genetics
MH  - Proto-Oncogene Proteins/genetics
OTO - NOTNLM
OT  - ALK
OT  - Cytology smear
OT  - Lung adenocarcinoma
OT  - Multiplex FISH
OT  - ROS1
EDAT- 2022/02/13 06:00
MHDA- 2022/04/21 06:00
CRDT- 2022/02/12 05:28
PHST- 2021/12/11 00:00 [received]
PHST- 2021/12/28 00:00 [revised]
PHST- 2022/01/05 00:00 [accepted]
PHST- 2022/02/13 06:00 [pubmed]
PHST- 2022/04/21 06:00 [medline]
PHST- 2022/02/12 05:28 [entrez]
AID - S2213-2945(22)00001-1 [pii]
AID - 10.1016/j.jasc.2022.01.001 [doi]
PST - ppublish
SO  - J Am Soc Cytopathol. 2022 May-Jun;11(3):154-164. doi: 10.1016/j.jasc.2022.01.001. 
      Epub 2022 Jan 13.