PMID- 35149417
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220315
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 212
DP  - 2022 Apr 1
TI  - Analysis of heparinase derived LMWH products using a MHC 2D LC system linked to 
      Q-TOF MS.
PG  - 114616
LID - S0731-7085(22)00037-1 [pii]
LID - 10.1016/j.jpba.2022.114616 [doi]
AB  - Low molecular weight heparins (LMWHs), depolymerized from unfractionated heparin 
      (UFH), are widely used as anticoagulant drugs in clinic. The variable degradation 
      methods result the different types of LMWHs, such as enoxaparin prepared by 
      alkaline degradation following benzylation and nadroparin degraded by nitrous 
      acid and subsequent reduction. They have different anticoagulant activities, 
      molecular weight and special oligosaccharide sequences. Oligosaccharide analysis 
      of the heparinase-catalyzed digestion products of heparin and LMWHs is an 
      important way to explore the fine structural composition. In this work, a 
      MHC-2D-LC-MS system using SAX followed by SEC and tandem to MS was applied to 
      analyze the heparinase-products of LMWHs. 15 components of enoxaparin and 20 
      components of nadroparin were separated and unambiguously characterized with mass 
      spectrum, including eight common disaccharides, and the special structural 
      domains resistant to enzyme digestion which have the 3-O sulfated residue and/or 
      characteristic terminal residues and the linkage region tetrasaccharides.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Chen, Lei
AU  - Chen L
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China.
FAU - Zhu, Wen
AU  - Zhu W
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China.
FAU - Yan, Na
AU  - Yan N
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China.
FAU - Guo, Yan
AU  - Guo Y
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China.
FAU - Yi, Lin
AU  - Yi L
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China.
FAU - Ouyang, Yilan
AU  - Ouyang Y
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China. Electronic address: y_ouyang@suda.edu.cn.
FAU - Zhang, Zhenqing
AU  - Zhang Z
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, 
      Suzhou, Jiangsu 215021, China. Electronic address: z_zhang@suda.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220129
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Anticoagulants)
RN  - 0 (Enoxaparin)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
RN  - EC 4.2.2.7 (Heparin Lyase)
SB  - IM
MH  - Anticoagulants/chemistry
MH  - Enoxaparin/chemistry
MH  - *Heparin/analysis
MH  - Heparin Lyase/metabolism
MH  - *Heparin, Low-Molecular-Weight/analysis
OTO - NOTNLM
OT  - Disaccharide
OT  - Linkage region
OT  - Low molecular weight heparin
OT  - Oligosaccharide
OT  - Structural elucidation
OT  - Two-dimensional liquid chromatography
EDAT- 2022/02/13 06:00
MHDA- 2022/03/16 06:00
CRDT- 2022/02/12 05:34
PHST- 2021/08/19 00:00 [received]
PHST- 2021/11/01 00:00 [revised]
PHST- 2021/11/02 00:00 [accepted]
PHST- 2022/02/13 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2022/02/12 05:34 [entrez]
AID - S0731-7085(22)00037-1 [pii]
AID - 10.1016/j.jpba.2022.114616 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2022 Apr 1;212:114616. doi: 10.1016/j.jpba.2022.114616. Epub 
      2022 Jan 29.