PMID- 35150830
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220406
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 122
DP  - 2022 Mar
TI  - Paired miRNA and RNA sequencing provides a first insight into molecular defense 
      mechanisms of Scapharca broughtonii during ostreid herpesvirus-1 infection.
PG  - 225-233
LID - S1050-4648(22)00064-X [pii]
LID - 10.1016/j.fsi.2022.02.004 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) infection caused mortalities with relevant 
      economic losses in bivalve aquaculture industry worldwide. Initially described as 
      an oyster pathogen, OsHV-1 can infect other bivalve species, like the blood clam 
      Scapharca broughtonii. However, at present, little is known about the molecular 
      interactions during OsHV-1 infection in the blood clam. We produced paired miRNA 
      and total RNA-seq data to investigate the blood clam transcriptional changes from 
      0 to 72 h after experimental infection with OsHV-1. High-throughput miRNA 
      sequencing of 24 libraries revealed 580 conserved and 270 new blood clam miRNAs, 
      whereas no genuine miRNA was identified for OsHV-1. Total 88-203 differently 
      expressed miRNAs were identified per time point, mostly up-regulated and mainly 
      targeting metabolic pathways. Most of the blood clam mRNAs, in contrast, were 
      down-regulated up to 60 h post-injection, with the trend analysis revealing the 
      activation of immune genes only when comparing the early and latest stage of 
      infection. Taken together, paired short and long RNA data suggested a 
      miRNA-mediated down-regulation of host metabolic and energetic processes as a 
      possible antiviral strategy during early infection stages, whereas antiviral 
      pathways appeared upregulated only at late infection.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Bai, Chang-Ming
AU  - Bai CM
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, 
      Qingdao, 266237, China.
FAU - Zhang, Xiang
AU  - Zhang X
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China; College of Fisheries, Tianjin Agriculture University, Tianjin, 
      300380, China.
FAU - Venier, Paola
AU  - Venier P
AD  - Department of Biology, University of Padova, 35121, Padova, Italy.
FAU - Gu, Li
AU  - Gu L
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China; College of Fisheries, Tianjin Agriculture University, Tianjin, 
      300380, China.
FAU - Li, Ya-Nan
AU  - Li YN
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China.
FAU - Wang, Chong-Ming
AU  - Wang CM
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, 
      Qingdao, 266237, China.
FAU - Xin, Lu-Sheng
AU  - Xin LS
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 
      266071, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, 
      Qingdao, 266237, China. Electronic address: xinls@ysfri.ac.cn.
FAU - Rosani, Umberto
AU  - Rosani U
AD  - Department of Biology, University of Padova, 35121, Padova, Italy. Electronic 
      address: umberto.rosani@unipd.it.
LA  - eng
PT  - Journal Article
DEP - 20220209
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (MicroRNAs)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - *Crassostrea/genetics
MH  - DNA Viruses/physiology
MH  - Defense Mechanisms
MH  - *Herpesviridae/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - *Scapharca/genetics
MH  - Sequence Analysis, RNA
OTO - NOTNLM
OT  - Aquaculture
OT  - Blood clam
OT  - OsHV-1
OT  - RNA-seq
OT  - miRNA
EDAT- 2022/02/13 06:00
MHDA- 2022/04/07 06:00
CRDT- 2022/02/12 20:10
PHST- 2021/11/19 00:00 [received]
PHST- 2022/01/29 00:00 [revised]
PHST- 2022/02/02 00:00 [accepted]
PHST- 2022/02/13 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2022/02/12 20:10 [entrez]
AID - S1050-4648(22)00064-X [pii]
AID - 10.1016/j.fsi.2022.02.004 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2022 Mar;122:225-233. doi: 10.1016/j.fsi.2022.02.004. 
      Epub 2022 Feb 9.