PMID- 35152180
OWN - NLM
STAT- MEDLINE
DCOM- 20220415
LR  - 20220415
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 60
DP  - 2022 Apr
TI  - Generation of human induced pluripotent stem cell lines encoding for genetically 
      encoded calcium indicators RCaMP1h and GCaMP6f.
PG  - 102697
LID - S1873-5061(22)00046-0 [pii]
LID - 10.1016/j.scr.2022.102697 [doi]
AB  - Calcium plays a key role in cardiomyocytes (CMs) for the translation of the 
      electrical impulse of an action potential into contraction forces. A rapid, 
      not-invasive fluorescence imaging technology allows for the monitoring of calcium 
      transients in human induced pluripotent stem cell derived-cardiomyocytes 
      (hiPSC-CMs) to investigate the cardiac electrophysiology in vitro and after cell 
      transplantation in vivo. The genetically encoded calcium indicators (GECIs) 
      GCaMP6f or RCaMP1h were successfully transfected in the previously established 
      hiPSC line MHHi001-A, together with a cardiac specific antibiotic selection 
      cassette facilitating the monitoring of the calcium handling in highly pure 
      populations of hiPSC-CMs.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Ricci Signorini, Maria Elena
AU  - Ricci Signorini ME
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Szepes, Monika
AU  - Szepes M
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Melchert, Anna
AU  - Melchert A
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Bakar, Mine
AU  - Bakar M
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Merkert, Sylvia
AU  - Merkert S
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Haase, Alexandra
AU  - Haase A
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Gohring, Gudrun
AU  - Gohring G
AD  - Department of Human Genetics, Hannover Medical School, Germany.
FAU - Martin, Ulrich
AU  - Martin U
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany.
FAU - Gruh, Ina
AU  - Gruh I
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), 
      Hannover Medical School, Germany. Electronic address: gruh.ina@mh-hannover.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220202
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials
MH  - Calcium/metabolism
MH  - Cell Differentiation
MH  - Humans
MH  - *Induced Pluripotent Stem Cells/metabolism
MH  - Myocytes, Cardiac/metabolism
EDAT- 2022/02/14 06:00
MHDA- 2022/04/16 06:00
CRDT- 2022/02/13 20:36
PHST- 2021/12/08 00:00 [received]
PHST- 2022/01/29 00:00 [accepted]
PHST- 2022/02/14 06:00 [pubmed]
PHST- 2022/04/16 06:00 [medline]
PHST- 2022/02/13 20:36 [entrez]
AID - S1873-5061(22)00046-0 [pii]
AID - 10.1016/j.scr.2022.102697 [doi]
PST - ppublish
SO  - Stem Cell Res. 2022 Apr;60:102697. doi: 10.1016/j.scr.2022.102697. Epub 2022 Feb 
      2.