PMID- 35153496
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220501
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 15
DP  - 2022
TI  - Decreased Levels of miR-126 and miR-132 in Plasma and Vitreous Humor of 
      Non-Proliferative Diabetic Retinopathy Among Subjects with Type-2 Diabetes 
      Mellitus.
PG  - 345-358
LID - 10.2147/DMSO.S346097 [doi]
AB  - PURPOSE: Diabetic retinopathy (DR), the leading cause of blindness among working 
      adults, is an urgent public health problem as diabetes mellitus (DM) is 
      increasing at an alarming rate. Hyperglycemia-induced endothelial dysfunction is 
      the principal contributing factor leading to the development of microangiopathy. 
      Altered levels of microRNA (miR), the negative regulator of protein-coding genes, 
      have been observed and considered to be markers for DR. Present study aimed to 
      find out whether miR levels in plasma could be effective biomarkers to 
      differentiate between type 2 diabetes mellitus (T2DM) with non-proliferative 
      retinopathy (NPDR) from T2DM with no-DR (DNR). METHODS: We recruited 50 T2DM 
      subjects comprising 31 NPDR and 19 DNR individuals. Surrogate markers of systemic 
      oxidative stress and vascular endothelial growth factor (VEGF) were measured in 
      plasma. Levels of miR-126 and miR-132 were determined in plasma and vitreous 
      fluid using real-time PCR. RESULTS: We observed that levels of miR-126 and 
      miR-132 were decreased in NPDR subjects in comparison to DNR. Plasma levels of 
      miRs were inversely correlated with secreted levels of VEGF and oxidative stress 
      marker. The levels of these miRs showed discriminating ability between NPDR and 
      DNR. CONCLUSION: Circulating miRs 126 and 132 in plasma or vitreous may serve as 
      biomarkers for early diabetic retinopathy risk prediction, provided validated in 
      a larger cohort and other forms of retinal vasculopathy or retinopathy in the 
      future.
CI  - (c) 2022 Pramanik et al.
FAU - Pramanik, Subhasish
AU  - Pramanik S
AD  - Department of Endocrinology & Metabolism, Institute of Post Graduate Medical 
      Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.
FAU - Saha, Chinmay
AU  - Saha C
AD  - Department of Endocrinology & Metabolism, Institute of Post Graduate Medical 
      Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.
AD  - Genome Science, School of Interdisciplinary Studies, University of Kalyani, 
      Nadia, 741235, West Bengal, India.
FAU - Chowdhury, Subhankar
AU  - Chowdhury S
AD  - Department of Endocrinology & Metabolism, Institute of Post Graduate Medical 
      Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.
FAU - Bose, Chiranjit
AU  - Bose C
AD  - Department of Endocrinology & Metabolism, Institute of Post Graduate Medical 
      Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.
FAU - Bhattacharyya, Nitai P
AU  - Bhattacharyya NP
AD  - Department of Endocrinology & Metabolism, Institute of Post Graduate Medical 
      Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.
FAU - Mondal, Lakshmi Kanta
AU  - Mondal LK
AUID- ORCID: 0000-0003-0057-0778
AD  - Department of Ophthalmology, Regional Institute of Ophthalmology, Medical College 
      Campus, Kolkata, 700 073, West Bengal, India.
LA  - eng
PT  - Journal Article
DEP - 20220204
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC8823438
OTO - NOTNLM
OT  - diabetic retinopathy
OT  - malondialdehyde
OT  - microRNA-126
OT  - microRNA-132
OT  - oxidative stress
OT  - vascular endothelial growth factor
COIS- The authors declare no conflicts of interest for this work.
EDAT- 2022/02/15 06:00
MHDA- 2022/02/15 06:01
PMCR- 2022/02/04
CRDT- 2022/02/14 05:28
PHST- 2021/11/02 00:00 [received]
PHST- 2022/01/24 00:00 [accepted]
PHST- 2022/02/14 05:28 [entrez]
PHST- 2022/02/15 06:00 [pubmed]
PHST- 2022/02/15 06:01 [medline]
PHST- 2022/02/04 00:00 [pmc-release]
AID - 346097 [pii]
AID - 10.2147/DMSO.S346097 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2022 Feb 4;15:345-358. doi: 10.2147/DMSO.S346097. 
      eCollection 2022.