PMID- 35154103
OWN - NLM
STAT- MEDLINE
DCOM- 20220303
LR  - 20220303
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Molecular Identification of Nocardia seriolae and Comparative Analysis of Spleen 
      Transcriptomes of Hybrid Snakehead (Channa maculata Female x Channa argus Male) 
      With Nocardiosis Disease.
PG  - 778915
LID - 10.3389/fimmu.2022.778915 [doi]
LID - 778915
AB  - Hybrid snakehead (Channa maculata female x Channa argus male) is a new freshwater 
      aquaculture fish species in southern China. During intensive aquaculture, hybrid 
      snakeheads are often infected by Nocardia seriolae. In this study, hybrid 
      snakehead infected suspiciously by N. seriolae in an artificial breeding pond 
      were examined. Diseased hybrid snakeheads swam slowly without food intake, and 
      the clinical symptoms included skin wound, anal swelling and ascites, and white 
      granulomatous in liver, spleen, and kidney of fish. Through bacterial isolation, 
      16S rDNA sequencing, fluorescence in situ hybridization (FISH) and artificial 
      infection experiment, the pathogen was identified as N. seriolae. Furthermore, 
      the spleen samples from diseased and healthy male hybrid snakeheads in the same 
      pond were used for RNA-Seq analysis. A total of 3,512 unique transcripts 
      (unigenes) were identified as differentially expressed genes (DEGs), and 1,886 of 
      them were up-regulated in diseased fish. The expression patterns of 20 DEGs were 
      verified by quantitative polymerase chain reaction (qPCR). Several immune-related 
      pathways and many immune-related genes were identified. qPCR results showed that 
      the expression patterns of immune-related genes in the liver and kidney of 
      diseased fish were comparable to that in the spleen. This study provides 
      deep-sequencing data of hybrid snakehead spleen and will help understand the 
      immune response of hybrid snakehead to N. seriolae. It is also helpful for the 
      biomarker screening of fish-borne Nocardia spp. and the breeding of 
      nocardiosis-resistant fish species.
CI  - Copyright (c) 2022 Zhang, Zhang, Dong and Wang.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Fisheries College, Guangdong Ocean University, Zhanjiang, China.
FAU - Zhang, Hairui
AU  - Zhang H
AD  - Zhongshan Ronghai Aquaculture Co. Ltd., Zhongshan, China.
FAU - Dong, Zhongdian
AU  - Dong Z
AD  - Fisheries College, Guangdong Ocean University, Zhanjiang, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Fisheries College, Guangdong Ocean University, Zhanjiang, China.
AD  - Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf 
      University, Qinzhou, China.
AD  - Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic 
      Economic Animals, Guangdong Ocean University, Zhanjiang, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220127
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Bacterial Proteins)
RN  - Nocardia seriolae
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Bacterial Proteins/immunology
MH  - China
MH  - Female
MH  - Fish Diseases/immunology/microbiology
MH  - Fishes/*genetics/immunology
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nocardia/classification/*genetics/immunology/*isolation & purification
MH  - Nocardia Infections/*veterinary
MH  - *Spleen
MH  - *Transcriptome
PMC - PMC8828968
OTO - NOTNLM
OT  - Nocardia seriolae
OT  - RNA-seq
OT  - fish
OT  - hybrid snakehead
OT  - immune response
COIS- Author HZ is employed by Zhongshan Ronghai aquaculture Co. Ltd. The remaining 
      authors declare that the research was conducted in the absence of any commercial 
      or financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2022/02/15 06:00
MHDA- 2022/03/04 06:00
PMCR- 2022/01/01
CRDT- 2022/02/14 05:30
PHST- 2021/09/17 00:00 [received]
PHST- 2022/01/10 00:00 [accepted]
PHST- 2022/02/14 05:30 [entrez]
PHST- 2022/02/15 06:00 [pubmed]
PHST- 2022/03/04 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.778915 [doi]
PST - epublish
SO  - Front Immunol. 2022 Jan 27;13:778915. doi: 10.3389/fimmu.2022.778915. eCollection 
      2022.