PMID- 35154214 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220216 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 12 DP - 2021 TI - Variation of Chromosome Composition in a Full-Sib Population Derived From 2x x 3x Interploidy Cross of Populus. PG - 816946 LID - 10.3389/fpls.2021.816946 [doi] LID - 816946 AB - Interploidy cross commonly results in complex chromosome number and structural variations. In our previous study, a progeny with segregated ploidy levels was produced by an interploidy cross between diploid female parent Populus tomentosa x Populus bolleana clone TB03 and triploid male parent Populus alba x Populus berolinensis 'Yinzhong'. However, the chromosome compositions of aneuploid genotypes in the progeny were still unclear. In the present study, a microsatellite DNA allele counting-peak ratios (MAC-PR) method was employed to analyze allelic configurations of each genotype to clarify their chromosome compositions, while 45S rDNA fluorescence in situ hybridization (FISH) analysis was used to reveal the mechanism of chromosome number variation. Based on the MAC-PR analysis of 47 polymorphic simple sequence repeat (SSR) markers distributed across all 19 chromosomes of Populus, both chromosomal number and structural variations were detected for the progeny. In the progeny, 26 hypo-triploids, 1 hyper-triploid, 16 hypo-tetraploids, 10 tetraploids, and 5 hyper-tetraploids were found. A total of 13 putative structural variation events (duplications and/or deletions) were detected in 12 genotypes, involved in chromosomes 3, 6, 7, 14, 15, 16, and 18. The 46.2% (six events) structural variation events occurred on chromosome 6, suggesting that there probably is a chromosome breakpoint near the SSR loci of chromosome 6. Based on calculation of the allelic information, the transmission of paternal heterozygosity in the hypo-triploids, hyper-triploid, hypo-tetraploids, tetraploids, and hyper-tetraploids were 0.748, 0.887, 0.830, 0.833, and 0.836, respectively, indicating that the viable pollen gains of the male parent 'Yinzhong' were able to transmit high heterozygosity to progeny. Furthermore, 45S rDNA-FISH analysis showed that specific-chromosome segregation feature during meiosis and chromosome appointment in normal and fused daughter nuclei of telophase II of 'Yinzhong,' which explained that the formation of aneuploids and tetraploids in the progeny could be attributed to imbalanced meiotic chromosomal segregation and division restitution of 'Yinzhong,' The data of chromosomal composition and structural variation of each aneuploid in the full-sib progeny of TB03 x 'Yinzhong' lays a foundation for analyzing mechanisms of trait variation relying on chromosome or gene dosages in Populus. CI - Copyright (c) 2022 Zhong, Zheng, Xue, Wang, Zhang, Li and Wang. FAU - Zhong, Yu-Hang AU - Zhong YH AD - National Engineering Research Center of Tree Breeding and Ecological Remediation, Beijing Forestry University, Beijing, China. AD - Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, China. AD - The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China. AD - College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. FAU - Zheng, Yun-Fei AU - Zheng YF AD - Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, China. AD - The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China. AD - College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. FAU - Xue, Yin-Xuan AU - Xue YX AD - National Engineering Research Center of Tree Breeding and Ecological Remediation, Beijing Forestry University, Beijing, China. AD - Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, China. AD - The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China. AD - College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. FAU - Wang, Lv-Ji AU - Wang LJ AD - National Engineering Research Center of Tree Breeding and Ecological Remediation, Beijing Forestry University, Beijing, China. AD - Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, China. AD - The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China. AD - College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. FAU - Zhang, Jin-Wang AU - Zhang JW AD - Forestry and Grassland Research Institute of Tongliao City, Tongliao, China. FAU - Li, Dai-Li AU - Li DL AD - Beijing Institute of Landscape Architecture, Beijing, China. FAU - Wang, Jun AU - Wang J AD - National Engineering Research Center of Tree Breeding and Ecological Remediation, Beijing Forestry University, Beijing, China. AD - Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, China. AD - The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China. AD - College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. LA - eng PT - Journal Article DEP - 20220126 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC8825477 OTO - NOTNLM OT - 45S rDNA-FISH OT - Populus OT - SSR karyotypic analysis OT - aneuploidy OT - chromosome composition OT - heterozygosity transmission OT - interploidy cross COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/02/15 06:00 MHDA- 2022/02/15 06:01 PMCR- 2021/01/01 CRDT- 2022/02/14 05:30 PHST- 2021/11/17 00:00 [received] PHST- 2021/12/27 00:00 [accepted] PHST- 2022/02/14 05:30 [entrez] PHST- 2022/02/15 06:00 [pubmed] PHST- 2022/02/15 06:01 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2021.816946 [doi] PST - epublish SO - Front Plant Sci. 2022 Jan 26;12:816946. doi: 10.3389/fpls.2021.816946. eCollection 2021.