PMID- 35154592
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220501
IS  - 2008-126X (Print)
IS  - 2228-7442 (Electronic)
IS  - 2008-126X (Linking)
VI  - 12
IP  - 4
DP  - 2021 Jul-Aug
TI  - Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration 
      Model.
PG  - 523-532
LID - 10.32598/bcn.2021.12.4.2230.1 [doi]
AB  - INTRODUCTION: Retinal Pigment Epithelium (RPE) layer deterioration is a leading 
      cause of Age-Related Macular Degeneration (AMD), i.e., the most significant 
      reason for irreversible blindness. The present study aimed to track the 
      Neurosphere-Derived (NS) from Bone Marrow Stromal Stem Cells (BMSCs) grafted into 
      the sub-retinal space (destruction of the RPE layer by sodium iodate). METHODS: 
      RPE degeneration model was performed using the injection of 5% sodium iodate 
      performed in the retro-orbital sinus of Wistar rats. BMSCs were extracted from 
      the examined rat femur and induced into NS, using EGF, bFGF, and B27. BrdU-NS 
      labeled cells were transplanted into the sub-retinal space. For detecting BMSCs 
      and NS markers, immunocytochemistry was performed. Moreover, immunohistochemical 
      was conducted for tracking the transplanted cells in the RPE and sensory retina. 
      RESULTS: The immunocytochemistry of BMSCs cells displayed the expression of 
      mesenchymal stem cells markers (CD90; 99%+/-1), CD166 (98%+/-2), CD44 (99%+/-1). 
      Additionally, the expression of neural lineage markers in NS, such as SOX2, OCT4, 
      Nanog, Nestin, and Neurofilaments (68, 160, 200) revealed the differentiation 
      from BMSCs. Tracking BrdU-NS labeled suggested these aggregations in most layers 
      of the retina. CONCLUSION: Our study data indicated that BMSCs derived 
      neurosphere had the potential to migrate in injured retinal and integrate into 
      the neurosensory retina. These data can be useful in finding safe cells for 
      replacement therapy in AMD.
CI  - Copyright(c) 2021 Iranian Neuroscience Society.
FAU - Kadkhodaeian, Hamid Aboutaleb
AU  - Kadkhodaeian HA
AUID- ORCID: 0000-0002-9736-9722
AD  - Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, 
      Semnan, Iran.
AD  - Department of Anatomical Sciences, School of Medicine, Semnan University of 
      Medical Sciences, Semnan, Iran.
FAU - Salati, Amir
AU  - Salati A
AUID- ORCID: 0000-0002-6840-1527
AD  - Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, 
      Semnan, Iran.
AD  - Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, 
      Semnan University of Medical Sciences, Semnan, Iran.
FAU - Ansari, Mojtaba
AU  - Ansari M
AUID- ORCID: 0000-0002-7312-8262
AD  - Department of Biomedical Engineering, University of Meybod, Meybod, Yazd, Iran.
FAU - Taghdiri Nooshabadi, Vajihe
AU  - Taghdiri Nooshabadi V
AUID- ORCID: 0000-0001-7762-715X
AD  - Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, 
      Semnan University of Medical Sciences, Semnan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210701
PL  - Iran
TA  - Basic Clin Neurosci
JT  - Basic and clinical neuroscience
JID - 101575211
PMC - PMC8817176
OTO - NOTNLM
OT  - Age-Related macular degeneration
OT  - Bone marrow stromal stem cells
OT  - Neurosphere cells
OT  - Sodium iodate
COIS- Conflict of interest The authors declared no conflicts of interest.
EDAT- 2022/02/15 06:00
MHDA- 2022/02/15 06:01
PMCR- 2021/07/01
CRDT- 2022/02/14 05:32
PHST- 2019/11/26 00:00 [received]
PHST- 2020/09/30 00:00 [revised]
PHST- 2021/06/02 00:00 [accepted]
PHST- 2022/02/14 05:32 [entrez]
PHST- 2022/02/15 06:00 [pubmed]
PHST- 2022/02/15 06:01 [medline]
PHST- 2021/07/01 00:00 [pmc-release]
AID - BCN-12-523 [pii]
AID - 10.32598/bcn.2021.12.4.2230.1 [doi]
PST - ppublish
SO  - Basic Clin Neurosci. 2021 Jul-Aug;12(4):523-532. doi: 
      10.32598/bcn.2021.12.4.2230.1. Epub 2021 Jul 1.