PMID- 35155445
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220216
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 9
DP  - 2021
TI  - Segmentally Demineralized Cortical Bone With Stem Cell-Derived Matrix Promotes 
      Proliferation, Migration and Differentiation of Stem Cells in vitro.
PG  - 776884
LID - 10.3389/fcell.2021.776884 [doi]
LID - 776884
AB  - A recent study has shown that demineralized cortical bone (DCB) did not improve 
      the healing of tendon-bone interface. Considering that there is a gradient of 
      mineral content in the tendon-bone interface, we designed a segmentally 
      demineralized cortical bone (sDCB) scaffold with two different regions: 
      undemineralized cortical bone section within the scaffold (sDCB-B) and complete 
      demineralized cortical bone section within the scaffold (sDCB-D), to mimic the 
      natural structure of the tendon-bone interface. Furthermore, the extracellular 
      matrix (ECM) from tendon-derived stem cells (TDSCs) was used to modify the sDCB-D 
      region of sDCB to construct a novel scaffold (sDCB-ECM) for enhancing the 
      bioactivity of the sDCB-D. The surface topography, elemental distribution, 
      histological structure, and surface elastic modulus of the scaffold were observed 
      using scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier 
      transform infrared spectroscopy, histological staining and atomic force 
      microscopy. Cell proliferation of bone marrow mesenchymal stem cells (BMSCs) and 
      TDSCs cultured on scaffolds was evaluated using the Cell Counting kit-8, and cell 
      viability was assessed by Live/Dead cell staining. Cell morphology was detected 
      by fluorescent staining. The ability of the scaffolds to recruit stem cells was 
      tested using transwell migration assay. The expression levels of bone-, 
      cartilage- and tendon-related genes and proteins in stem cells were assessed by 
      the polymerase chain reaction and western blotting. Our results demonstrated that 
      there was a gradient of Ca and P elements in sDCB, and TDSC-derived ECM existed 
      on the surface of the sDCB-D region of sDCB. The sDCB-ECM could promote stem cell 
      proliferation and migration. Moreover, the sDCB-B region of sDCB-ECM could 
      stimulate osteogenic and chondrogenic differentiation of BMSCs, and the 
      sDCB-D-ECM region of sDCB-ECM could stimulate chondrogenic and tenogenic 
      differentiation of TDSCs when compared to DCB. Our study indicated that sDCB-ECM 
      might be a potential bioscaffold to enhance the tendon-bone interface 
      regeneration.
CI  - Copyright (c) 2022 He, Ning, Hu, Yao, Cui, Ding, Luo and Qin.
FAU - He, Shu-Kun
AU  - He SK
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
AD  - Department of Orthopedics, West China Hospital, Orthopedic Research Institute, 
      Sichuan University, Chengdu, China.
AD  - Department of Orthopedics, First Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, China.
FAU - Ning, Liang-Ju
AU  - Ning LJ
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
FAU - Hu, Ruo-Nan
AU  - Hu RN
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
FAU - Yao, Xuan
AU  - Yao X
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
AD  - Department of Clinical Hematology, Faculty of Laboratory Medicine, Army Medical 
      University, Chongqing, China.
FAU - Cui, Jing
AU  - Cui J
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
FAU - Ding, Wei
AU  - Ding W
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
FAU - Luo, Jing-Cong
AU  - Luo JC
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
FAU - Qin, Ting-Wu
AU  - Qin TW
AD  - Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy, West China Hospital, Orthopedic Research Institute, Sichuan 
      University, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20220126
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC8826562
OTO - NOTNLM
OT  - differentiation
OT  - migration
OT  - proliferation
OT  - segmentally demineralized cortical bone
OT  - stem cell-derived matrix
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/02/15 06:00
MHDA- 2022/02/15 06:01
PMCR- 2021/01/01
CRDT- 2022/02/14 05:35
PHST- 2021/09/14 00:00 [received]
PHST- 2021/12/31 00:00 [accepted]
PHST- 2022/02/14 05:35 [entrez]
PHST- 2022/02/15 06:00 [pubmed]
PHST- 2022/02/15 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 776884 [pii]
AID - 10.3389/fcell.2021.776884 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2022 Jan 26;9:776884. doi: 10.3389/fcell.2021.776884. 
      eCollection 2021.