PMID- 35157735 OWN - NLM STAT- MEDLINE DCOM- 20220307 LR - 20220307 IS - 1553-7374 (Electronic) IS - 1553-7366 (Print) IS - 1553-7366 (Linking) VI - 18 IP - 2 DP - 2022 Feb TI - SARS-CoV-2-specific T cells generated for adoptive immunotherapy are capable of recognizing multiple SARS-CoV-2 variants. PG - e1010339 LID - 10.1371/journal.ppat.1010339 [doi] LID - e1010339 AB - Adoptive T-cell immunotherapy has provided promising results in the treatment of viral complications in humans, particularly in the context of immunocompromised patients who have exhausted all other clinical options. The capacity to expand T cells from healthy immune individuals is providing a new approach to anti-viral immunotherapy, offering rapid off-the-shelf treatment with tailor-made human leukocyte antigen (HLA)-matched T cells. While most of this research has focused on the treatment of latent viral infections, emerging evidence that SARS-CoV-2-specific T cells play an important role in protection against COVID-19 suggests that the transfer of HLA-matched allogeneic off-the-shelf virus-specific T cells could provide a treatment option for patients with active COVID-19 or at risk of developing COVID-19. We initially screened 60 convalescent individuals and based on HLA typing and T-cell response profile, 12 individuals were selected for the development of a SARS-CoV-2-specific T-cell bank. We demonstrate that these T cells are specific for up to four SARS-CoV-2 antigens presented by a broad range of both HLA class I and class II alleles. These T cells show consistent functional and phenotypic properties, display cytotoxic potential against HLA-matched targets and can recognize HLA-matched cells infected with different SARS-CoV-2 variants. These observations demonstrate a robust approach for the production of SARS-CoV-2-specific T cells and provide the impetus for the development of a T-cell repository for clinical assessment. FAU - Panikkar, Archana AU - Panikkar A AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Lineburg, Katie E AU - Lineburg KE AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Raju, Jyothy AU - Raju J AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Chew, Keng Yih AU - Chew KY AD - School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia Queensland, Australia. FAU - Ambalathingal, George R AU - Ambalathingal GR AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Rehan, Sweera AU - Rehan S AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Swaminathan, Srividhya AU - Swaminathan S AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. AD - Faculty of Medicine, The University of Queensland, Herston, Queensland, Australia. FAU - Crooks, Pauline AU - Crooks P AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Le Texier, Laetitia AU - Le Texier L AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Beagley, Leone AU - Beagley L AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Best, Shannon AU - Best S AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Solomon, Matthew AU - Solomon M AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Matthews, Katherine K AU - Matthews KK AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Srihari, Sriganesh AU - Srihari S AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Neller, Michelle A AU - Neller MA AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. FAU - Short, Kirsty R AU - Short KR AD - School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia Queensland, Australia. AD - Australian Infectious Diseases Research Centre, The University of Queensland, St Lucia Queensland, Australia. FAU - Khanna, Rajiv AU - Khanna R AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. AD - Faculty of Medicine, The University of Queensland, Herston, Queensland, Australia. FAU - Smith, Corey AU - Smith C AUID- ORCID: 0000-0002-7550-9595 AD - QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Translational and Human Immunology Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia. AD - Faculty of Medicine, The University of Queensland, Herston, Queensland, Australia. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220214 PL - United States TA - PLoS Pathog JT - PLoS pathogens JID - 101238921 RN - 0 (Epitopes, T-Lymphocyte) RN - 0 (HLA Antigens) RN - SARS-CoV-2 variants SB - IM MH - Adult MH - Epitopes, T-Lymphocyte MH - Female MH - HEK293 Cells MH - HLA Antigens/*immunology MH - Humans MH - Immunophenotyping MH - *Immunotherapy, Adoptive MH - Male MH - Middle Aged MH - SARS-CoV-2/*immunology MH - T-Lymphocytes/*immunology MH - Young Adult PMC - PMC8880869 COIS- The authors have declared that no competing interests exist. EDAT- 2022/02/15 06:00 MHDA- 2022/03/08 06:00 PMCR- 2022/02/14 CRDT- 2022/02/14 17:11 PHST- 2021/12/17 00:00 [received] PHST- 2022/02/04 00:00 [accepted] PHST- 2022/02/25 00:00 [revised] PHST- 2022/02/15 06:00 [pubmed] PHST- 2022/03/08 06:00 [medline] PHST- 2022/02/14 17:11 [entrez] PHST- 2022/02/14 00:00 [pmc-release] AID - PPATHOGENS-D-21-02553 [pii] AID - 10.1371/journal.ppat.1010339 [doi] PST - epublish SO - PLoS Pathog. 2022 Feb 14;18(2):e1010339. doi: 10.1371/journal.ppat.1010339. eCollection 2022 Feb.