PMID- 35158353
OWN - NLM
STAT- MEDLINE
DCOM- 20220216
LR  - 20220216
IS  - 1423-0143 (Electronic)
IS  - 1420-4096 (Linking)
VI  - 47
IP  - 2
DP  - 2022
TI  - Circulating miRNAs in Type 2 Diabetic Patients with and without Albuminuria in 
      Malaysia.
PG  - 81-93
LID - 10.1159/000518866 [doi]
AB  - INTRODUCTION: Diabetic kidney disease (DKD) remains the leading cause of chronic 
      kidney disease. Dysregulation of circulating miRNAs has been reported, suggesting 
      their pathological roles in DKD. This study aimed to investigate differentially 
      expressed miRNAs in the sera of type 2 diabetes mellitus (T2DM) patients with and 
      without albuminuria in a selected Malaysian population. METHOD: Forty-one T2DM 
      patients on follow-up at a community clinic were divided into normo-(NA), 
      micro-(MIC), and macroalbuminuria (MAC) groups. Differential levels of miRNAs in 
      12 samples were determined using the pathway-focused (human fibrosis) miScript 
      miRNA qPCR array and was validated in 33 samples, using the miScript custom qPCR 
      array (CMIHS02742) (Qiagen GmbH, Hilden, Germany). RESULTS: Trends of 
      upregulation of 3 miRNAs in the serum, namely, miR-874-3p, miR-101-3p, and 
      miR-145-5p of T2DM patients with MAC compared to those with NA. Statistically 
      significant upregulation of miR-874-3p (p = 0.04) and miR-101-3p (p = 0.01) was 
      seen in validation cohort. Significant negative correlations between the 
      estimated glomerular filtration rate (eGFR) and miR-874-3p (p = 0.05), miR-101-3p 
      (p = 0.03), and miR-145-5p (p = 0.05) as well as positive correlation between 
      miR-874-3p and age (p = 0.03) were shown by Pearson's correlation coefficient 
      analysis. CONCLUSION: Upregulation of previously known miRNA, namely, miR-145-5p, 
      and possibly novel ones, namely, miR-874-3p and miR-101-3p in the serum of T2DM 
      patients, was found in this study. There was a significant correlation between 
      the eGFR and these miRNAs. The findings of this study have provided encouraging 
      evidence to further investigate the putative roles of these differentially 
      expressed miRNAs in DKD.
CI  - (c) 2022 The Author(s). Published by S. Karger AG, Basel.
FAU - Zahari Sham, Siti Yazmin
AU  - Zahari Sham SY
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
FAU - Ng, Chin Tat
AU  - Ng CT
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
FAU - Azwar, Shamin
AU  - Azwar S
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
FAU - Yip, Wai Kien
AU  - Yip WK
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
FAU - Abdullah, Maha
AU  - Abdullah M
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
FAU - Thevandran, Kalaiselvam
AU  - Thevandran K
AD  - Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra 
      Malaysia, Serdang, Malaysia.
FAU - Osman, Malina
AU  - Osman M
AD  - Department of Medical Microbiology and Parasitology, Faculty of Medicine and 
      Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
FAU - Seow, Heng Fong
AU  - Seow HF
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, Universiti 
      Putra Malaysia, Serdang, Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20220111
PL  - Switzerland
TA  - Kidney Blood Press Res
JT  - Kidney & blood pressure research
JID - 9610505
RN  - 0 (Biomarkers)
RN  - 0 (MIRN145 microRNA, human)
RN  - 0 (MIRN874 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Albuminuria
MH  - Biomarkers
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - Humans
MH  - Malaysia
MH  - *MicroRNAs
OTO - NOTNLM
OT  - Albuminuria
OT  - Diabetic kidney disease
OT  - Serum
OT  - Type 2 diabetes mellitus
OT  - miRNAs
EDAT- 2022/02/15 06:00
MHDA- 2022/02/17 06:00
CRDT- 2022/02/14 20:17
PHST- 2021/02/09 00:00 [received]
PHST- 2021/08/03 00:00 [accepted]
PHST- 2022/02/14 20:17 [entrez]
PHST- 2022/02/15 06:00 [pubmed]
PHST- 2022/02/17 06:00 [medline]
AID - 000518866 [pii]
AID - 10.1159/000518866 [doi]
PST - ppublish
SO  - Kidney Blood Press Res. 2022;47(2):81-93. doi: 10.1159/000518866. Epub 2022 Jan 
      11.