PMID- 35158945
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220219
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 14
IP  - 3
DP  - 2022 Jan 28
TI  - TFF1 in Aqueous Humor-A Potential New Biomarker for Retinoblastoma.
LID - 10.3390/cancers14030677 [doi]
LID - 677
AB  - Retinoblastoma (RB) is the most common childhood eye cancer. The expression of 
      trefoil factor family peptide 1 (TFF1), a small secreted peptide, has been 
      correlated with more advanced RB stages and it might be a promising new candidate 
      as a RB biomarker. The study presented addressed the question of if TFF1 is 
      detectable in aqueous humor (AH) of RB patients' eyes, providing easy 
      accessibility as a diagnostic and/or therapy accompanying predictive biomarker. 
      The TFF1 expression status of 15 retinoblastoma AH samples was investigated by 
      ELISA and Western blot analyses. The results were correlated with the TFF1 
      expression status in the tumor of origin and compared to TFF1 expression in 
      established corresponding primary tumor cell cultures and supernatants. Nine out 
      of fifteen AH patient samples exhibited TFF1 expression, which correlated well 
      with TFF1 levels of the original tumor. TFF1 expression in most of the 
      corresponding primary cell cultures reflects the levels of the original tumor, 
      although not all TFF1-expressing tumor cells seem to secret into the AH. 
      Together, our findings strongly suggest TFF1 as a reliable new RB biomarker.
FAU - Busch, Maike Anna
AU  - Busch MA
AUID- ORCID: 0000-0001-9651-031X
AD  - Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, 
      Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Haase, Andre
AU  - Haase A
AUID- ORCID: 0000-0002-4529-1396
AD  - Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, 
      Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Miroschnikov, Natalia
AU  - Miroschnikov N
AD  - Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, 
      Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Doege, Annika
AU  - Doege A
AD  - Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, 
      Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Biewald, Eva
AU  - Biewald E
AD  - Department of Ophthalmology, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Bechrakis, Nikolaos E
AU  - Bechrakis NE
AD  - Department of Ophthalmology, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Beier, Manfred
AU  - Beier M
AD  - Institute of Human Genetics, Medical Faculty, Heinrich-Heine University, 40225 
      Dusseldorf, Germany.
FAU - Kanber, Deniz
AU  - Kanber D
AD  - Institute of Human Genetics, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Lohmann, Dietmar
AU  - Lohmann D
AUID- ORCID: 0000-0002-2624-9889
AD  - Institute of Human Genetics, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Metz, Klaus
AU  - Metz K
AD  - Institute of Pathology, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
FAU - Dunker, Nicole
AU  - Dunker N
AUID- ORCID: 0000-0003-1273-0644
AD  - Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, 
      Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 
      Essen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20220128
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC8833755
OTO - NOTNLM
OT  - RB
OT  - TFF1
OT  - aqueous humor
OT  - biomarker
OT  - liquid biopsy
OT  - retinoblastoma
OT  - trefoil factor family peptides
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/02/16 06:01
PMCR- 2022/01/28
CRDT- 2022/02/15 01:03
PHST- 2022/01/04 00:00 [received]
PHST- 2022/01/24 00:00 [revised]
PHST- 2022/01/27 00:00 [accepted]
PHST- 2022/02/15 01:03 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/02/16 06:01 [medline]
PHST- 2022/01/28 00:00 [pmc-release]
AID - cancers14030677 [pii]
AID - cancers-14-00677 [pii]
AID - 10.3390/cancers14030677 [doi]
PST - epublish
SO  - Cancers (Basel). 2022 Jan 28;14(3):677. doi: 10.3390/cancers14030677.