PMID- 3515979
OWN - NLM
STAT- MEDLINE
DCOM- 19860507
LR  - 20191029
IS  - 8755-8920 (Print)
IS  - 8755-8920 (Linking)
VI  - 10
IP  - 2
DP  - 1986 Feb
TI  - Failure to identify HLA ABC and Dr antigens on human sperm.
PG  - 39-46
AB  - An indirect radiolabeled antiglobulin assay (IRAA), adsorption procedures, and an 
      indirect immunofluorescent assay using cytofluorometry (IFAA) failed to identify 
      HLA ABC or D/Dr antigens associated with untreated human sperm or human sperm 
      exposed to capacitating conditions. Two monoclonal clones against HLA ABC and two 
      clones against Dr antigens were utilized. Test results in the IRAA were not 
      different regardless of whether sperm from fertile men or human red blood cells 
      were employed in the IRAA. However, there were highly significant differences in 
      the IRAA between the HLA antigen expression of human sperm and leukocytes. If the 
      antihuman HLA monoclonal antibody was preadsorbed with 25 X 10(6) sperm, there 
      was no diminution in the ability of the adsorbed antibody to identify HLA 
      antigens on leukocytes; however, preadsorption with 2.5 X 10(6) leukocytes 
      reduced the activity of the monoclonal antihuman HLA antibody to levels 
      equivalent to background activity.
FAU - Haas, G G Jr
AU  - Haas GG Jr
FAU - Nahhas, F
AU  - Nahhas F
LA  - eng
GR  - HD-19908/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Reprod Immunol Microbiol
JT  - American journal of reproductive immunology and microbiology : AJRIM
JID - 8501543
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Coombs Test
MH  - Erythrocytes/immunology
MH  - HLA Antigens/*isolation & purification
MH  - HLA-DR Antigens
MH  - Histocompatibility Antigens Class II/*isolation & purification
MH  - Humans
MH  - Immunosorbent Techniques
MH  - Infertility, Male/immunology
MH  - Leukocytes/immunology
MH  - Male
MH  - Spermatozoa/*immunology
EDAT- 1986/02/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1986/02/01 00:00
PHST- 1986/02/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1986/02/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1986.tb00008.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol Microbiol. 1986 Feb;10(2):39-46. doi: 
      10.1111/j.1600-0897.1986.tb00008.x.