PMID- 35159838 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220219 IS - 2079-4991 (Print) IS - 2079-4991 (Electronic) IS - 2079-4991 (Linking) VI - 12 IP - 3 DP - 2022 Jan 30 TI - Plumeria alba-Mediated Green Synthesis of Silver Nanoparticles Exhibits Antimicrobial Effect and Anti-Oncogenic Activity against Glioblastoma U118 MG Cancer Cell Line. LID - 10.3390/nano12030493 [doi] LID - 493 AB - Plumeria alba (P. alba) is a small laticiferous tree with promising medicinal properties. Green synthesis of nanoparticles is eco-friendly, cost-effective, and non-hazardous compared to chemical and physical synthesis methods. Current research aiming to synthesize silver nanoparticles (AgNPs) from the leaf extract of P. alba (P- AgNPs) has described its physiochemical and pharmacological properties in recognition of its therapeutic potential as an anticancer and antimicrobial agent. These biogenic synthesized P-AgNPs were physiochemically characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM), X-ray diffractometry (XRD), and zeta potential analysis. Antimicrobial activity was investigated against Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Enterococcus faecalis, Bacillus subtilis, Streptococcus pneumoniae, Candida albicans, and Candida glabrata. Anticancer activity against glioblastoma U118 MG cancer lines was investigated using an MTT assay, and apoptosis activity was determined by flow cytometry. UV-visible spectroscopic analysis portrayed surface plasmon resonance at 403 nm of synthesized P-AgNPs, and FTIR suggested the presence of amines, alkanes, and phenol molecules that could be involved in reduction and capping processes during AgNPs formation. Synthesized particles were spherical in shape and poly-dispersed with an average particle size of 26.43 nm and a poly-dispersity index (PDI) of 0.25 with a zeta potential value of -24.6 mV, ensuring their stability. The lattice plane values confirm the crystalline nature as identified by XRD. These P-AgNPs exhibited potential antimicrobial activity against selected human pathogenic microbes. Additionally, the in vitro MTT assay results showed its effective anticancer activity against the glioma U118 MG cancer cell line with an IC(50) value of 9.77 microg/mL AgNPs by initiating apoptosis as identified by a staining study with flow cytometric Annexin V-Fluorescein Isothiocyanate (FITC) and Propidium Iodide (PI). Thus, P. alba AgNPs can be recommended for further pharmacological and other biological research. To conclude, the current investigation developed an eco-friendly AgNPs synthesis using P. alba leaf extract with potential cytotoxic and antibacterial capacity, which can therefore be recommended as a new strategy to treat different human diseases. FAU - Rudrappa, Muthuraj AU - Rudrappa M AUID- ORCID: 0000-0003-3406-1648 AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Rudayni, Hassan Ahmed AU - Rudayni HA AUID- ORCID: 0000-0003-4464-5989 AD - Department of Biology, College of Science, Imam Muhammad bin Saud Islamic University, Riyadh 11623, Saudi Arabia. FAU - Assiri, Rasha Assad AU - Assiri RA AD - Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia. FAU - Bepari, Asmatanzeem AU - Bepari A AUID- ORCID: 0000-0001-7633-2923 AD - Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia. FAU - Basavarajappa, Dhanyakumara Shivapoojar AU - Basavarajappa DS AUID- ORCID: 0000-0001-7043-0653 AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Nagaraja, Shashiraj Kariyellappa AU - Nagaraja SK AUID- ORCID: 0000-0003-4920-6459 AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Chakraborty, Bidhayak AU - Chakraborty B AUID- ORCID: 0000-0001-5296-0650 AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Swamy, Pallavi Sathyanarayana AU - Swamy PS AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Agadi, Shekappa Ningappa AU - Agadi SN AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. FAU - Niazi, Shaik Kalimulla AU - Niazi SK AUID- ORCID: 0000-0002-1954-7036 AD - Department of Preparatory Health Sciences, Riyadh Elm University, Riyadh 12611, Saudi Arabia. FAU - Nayaka, Sreenivasa AU - Nayaka S AUID- ORCID: 0000-0002-7339-5715 AD - Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India. LA - eng GR - Seed grant for research program (2021- 22/62)/Karnatak University, Dharwad, Karnataka, India/ PT - Journal Article DEP - 20220130 PL - Switzerland TA - Nanomaterials (Basel) JT - Nanomaterials (Basel, Switzerland) JID - 101610216 PMC - PMC8839720 OTO - NOTNLM OT - Apocynaceae OT - Plumeria alba OT - apoptosis OT - chemotherapeutics OT - cytotoxic OT - nano-antibiotics OT - nanotechnology COIS- The authors declare that there is no conflict of interest. EDAT- 2022/02/16 06:00 MHDA- 2022/02/16 06:01 PMCR- 2022/01/30 CRDT- 2022/02/15 01:06 PHST- 2021/12/05 00:00 [received] PHST- 2022/01/23 00:00 [revised] PHST- 2022/01/28 00:00 [accepted] PHST- 2022/02/15 01:06 [entrez] PHST- 2022/02/16 06:00 [pubmed] PHST- 2022/02/16 06:01 [medline] PHST- 2022/01/30 00:00 [pmc-release] AID - nano12030493 [pii] AID - nanomaterials-12-00493 [pii] AID - 10.3390/nano12030493 [doi] PST - epublish SO - Nanomaterials (Basel). 2022 Jan 30;12(3):493. doi: 10.3390/nano12030493.