PMID- 35160583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220219
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 14
IP  - 3
DP  - 2022 Feb 1
TI  - Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by 
      Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells.
LID - 10.3390/polym14030594 [doi]
LID - 594
AB  - The great variability of cancer types demands novel drugs with broad spectrum, 
      this is the case of Nisin, a polycyclic antibacterial peptide that recently has 
      been considered for prevention of cancer cells growth. As an accepted food 
      additive, this drug would be very useful for intestinal cancers, but the peptide 
      nature would make easier its degradation by digestion procedures. For that 
      reason, the aim of present study to investigate the protective effect of two 
      different beta-cyclodextrin-based nanosponges (carbonyl diimidazole and pyromellitic 
      dianhydride) and their anti-cancer enhancement effect of Nisin-Z encapsulated 
      with against colon cancer cells (HT-29). To extend its possible use, a comparison 
      with breast (MCF-7) cancer cell was carried out. The physicochemical properties, 
      loading efficiency, and release kinetics of Nisin complex with nanosponges were 
      studied. Then, tricin-SDS-PAGE electrophoresis was used to understand the effect 
      of NSs on stability of Nisin-Z in the presence of gastric peptidase pepsin. In 
      addition, the cytotoxicity and cell membrane damage of Nisin Z were evaluated by 
      using the MTT and LDH assay, which was complemented via Annexin-V/ Propidium 
      Iodide (PI) by using flowcytometry. CD-NS are able to complex Nisin-Z with an 
      encapsulation efficiency around 90%. A protective effect of Nisin-Z complexed 
      with CD-NSs was observed in presence of pepsin. An increase in the percentage of 
      apoptotic cells was observed when the cancer cells were exposed to Nisin Z 
      complexed with nanosponges. Interestingly, Nisin Z free and loaded on 
      PMDA/CDI-NSs is more selectively toxic towards HT-29 cells than MCF-7 cancer 
      cells. These results indicated that nanosponges might be good candidates to 
      protect peptides and deliver drugs against intestinal cancers.
FAU - Khazaei Monfared, Yousef
AU  - Khazaei Monfared Y
AUID- ORCID: 0000-0002-1171-8670
AD  - Dipartimento Di Chimica, Universita di Torino, Via P. Giuria 7, 10125 Torino, 
      Italy.
FAU - Mahmoudian, Mohammad
AU  - Mahmoudian M
AD  - Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166414766, 
      Iran.
FAU - Cecone, Claudio
AU  - Cecone C
AUID- ORCID: 0000-0002-2660-9522
AD  - Dipartimento Di Chimica, Universita di Torino, Via P. Giuria 7, 10125 Torino, 
      Italy.
FAU - Caldera, Fabrizio
AU  - Caldera F
AUID- ORCID: 0000-0003-2581-0555
AD  - Dipartimento Di Chimica, Universita di Torino, Via P. Giuria 7, 10125 Torino, 
      Italy.
FAU - Zakeri-Milani, Parvin
AU  - Zakeri-Milani P
AD  - Liver and Gastrointestinal Diseases Research Centre and Faculty of Pharmacy, 
      Tabriz University of Medical Sciences, Tabriz 5166414766, Iran.
FAU - Matencio, Adrian
AU  - Matencio A
AUID- ORCID: 0000-0001-8203-0673
AD  - Dipartimento Di Chimica, Universita di Torino, Via P. Giuria 7, 10125 Torino, 
      Italy.
FAU - Trotta, Francesco
AU  - Trotta F
AD  - Dipartimento Di Chimica, Universita di Torino, Via P. Giuria 7, 10125 Torino, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20220201
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC8840141
OTO - NOTNLM
OT  - HT-29
OT  - MCF-7
OT  - Nisin-Z stability
OT  - anti-cancer
OT  - beta-cyclodextrin-based nanosponges
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/02/16 06:01
PMCR- 2022/02/01
CRDT- 2022/02/15 01:08
PHST- 2021/10/07 00:00 [received]
PHST- 2021/11/19 00:00 [revised]
PHST- 2022/01/29 00:00 [accepted]
PHST- 2022/02/15 01:08 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/02/16 06:01 [medline]
PHST- 2022/02/01 00:00 [pmc-release]
AID - polym14030594 [pii]
AID - polymers-14-00594 [pii]
AID - 10.3390/polym14030594 [doi]
PST - epublish
SO  - Polymers (Basel). 2022 Feb 1;14(3):594. doi: 10.3390/polym14030594.