PMID- 35162026
OWN - NLM
STAT- MEDLINE
DCOM- 20220216
LR  - 20220219
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 22
IP  - 3
DP  - 2022 Feb 8
TI  - A Simple Yet Effective Preanalytical Strategy Enabling the Application of 
      Aptamer-Conjugated Gold Nanoparticles for the Colorimetric Detection of 
      Antibiotic Residues in Raw Milk.
LID - 10.3390/s22031281 [doi]
LID - 1281
AB  - The misuse of antibiotics in the cattle sector can lead to milk contamination, 
      with concomitant effects on the dairy industry and human health. Biosensors can 
      be applied in this field; however, the influence of the milk matrix on their 
      activity has been poorly studied in light of the preanalytical process. Herein, 
      aptamer-conjugated gold nanoparticles (nanoaptasensors) were investigated for the 
      colorimetric detection in raw milk of four antibiotics used in cattle. The effect 
      of milk components on the colorimetric response of the nanoaptasensors was 
      analyzed by following the selective aggregation of the nanoparticles, using the 
      absorption ratio A(520)/A(720). A preanalytical strategy was developed to apply 
      the nanoaptasensors to antibiotic-contaminated raw milk samples, which involves a 
      clarification step with Carrez reagents followed by the removal of cations 
      through dilution, chelation (EDTA) or precipitation (NaHCO(3)). The colorimetric 
      signals were detected in spiked samples at concentrations of antibiotics as low 
      as 0.25-fold the maximum residue limits (MRLs) for kanamycin (37.5 mug/L), 
      oxytetracycline (25 mug/L), sulfadimethoxine (6.25 mug/L) and ampicillin (1 mug/L), 
      according to European and Chilean legislation. Overall, we conclude that this 
      methodology holds potential for the semiquantitative analysis of antibiotic 
      residues in raw milk obtained directly from dairy farms.
FAU - Diaz-Garcia, Victor
AU  - Diaz-Garcia V
AUID- ORCID: 0000-0002-2343-4712
AD  - Facultad de Ingenieria y Tecnologia, Universidad San Sebastian, Sede Concepcion, 
      Concepcion 4080871, Chile.
FAU - Contreras-Trigo, Braulio
AU  - Contreras-Trigo B
AD  - Facultad de Ingenieria y Tecnologia, Universidad San Sebastian, Sede Concepcion, 
      Concepcion 4080871, Chile.
FAU - Rodriguez, Camila
AU  - Rodriguez C
AD  - Facultad de Ingenieria y Tecnologia, Universidad San Sebastian, Sede Concepcion, 
      Concepcion 4080871, Chile.
FAU - Coelho, Pablo
AU  - Coelho P
AUID- ORCID: 0000-0002-7501-7785
AD  - Facultad de Ingenieria y Tecnologia, Universidad San Sebastian, Sede Concepcion, 
      Concepcion 4080871, Chile.
FAU - Oyarzun, Patricio
AU  - Oyarzun P
AUID- ORCID: 0000-0001-7305-1632
AD  - Facultad de Ingenieria y Tecnologia, Universidad San Sebastian, Sede Concepcion, 
      Concepcion 4080871, Chile.
LA  - eng
GR  - PAI77180078/Agencia Nacional de Investigacion y Desarrollo/
GR  - Fondecyt Posdoctorado 3190734/Agencia Nacional de Investigacion y Desarrollo/
PT  - Journal Article
DEP - 20220208
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Aptamers, Nucleotide)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/analysis
MH  - *Aptamers, Nucleotide
MH  - Cattle
MH  - Colorimetry
MH  - Gold
MH  - Limit of Detection
MH  - *Metal Nanoparticles
MH  - Milk/chemistry
PMC - PMC8837955
OTO - NOTNLM
OT  - antibiotics
OT  - aptamer
OT  - clarification
OT  - gold nanoparticles
OT  - nanoaptasensor
OT  - preanalytical processing
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/02/17 06:00
PMCR- 2022/02/08
CRDT- 2022/02/15 01:12
PHST- 2021/12/05 00:00 [received]
PHST- 2021/12/29 00:00 [revised]
PHST- 2021/12/29 00:00 [accepted]
PHST- 2022/02/15 01:12 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/02/17 06:00 [medline]
PHST- 2022/02/08 00:00 [pmc-release]
AID - s22031281 [pii]
AID - sensors-22-01281 [pii]
AID - 10.3390/s22031281 [doi]
PST - epublish
SO  - Sensors (Basel). 2022 Feb 8;22(3):1281. doi: 10.3390/s22031281.