PMID- 3516311
OWN - NLM
STAT- MEDLINE
DCOM- 19860613
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 369
IP  - 1-2
DP  - 1986 Mar 26
TI  - N-methylaspartate: an effective tool for lesioning basal forebrain cholinergic 
      neurons of the rat.
PG  - 377-82
AB  - The ability of the excitotoxin, N-methyl-D,L-aspartic acid (NMA), to destroy 
      basal forebrain cholinergic (BFC) neurons was evaluated. NMA (100 nmol) was 
      directly injected into the peripallidum, a region containing a proportionately 
      large number of cortically-projecting BFC neurons. Cholineacetyltransferase 
      (ChAT) activity 10 days later was markedly and significantly reduced (up to 62%) 
      in the cortex ipsilateral to the lesion. NMA induced a focal lesion affecting BFC 
      neurons without damaging axons of passage or causing lesions distant from the 
      site of injection. ChAT immunohistochemistry (IHC) was used to directly 
      demonstrate loss of ChAT-positive neurons from the lesion site. This loss 
      persisted at all survival times examined, from 2 days to 7.5 months 
      post-injection.
FAU - Stewart, G R
AU  - Stewart GR
FAU - Price, M
AU  - Price M
FAU - Olney, J W
AU  - Olney JW
FAU - Hartman, B K
AU  - Hartman BK
FAU - Cozzari, C
AU  - Cozzari C
LA  - eng
GR  - AG05681/AG/NIA NIH HHS/United States
GR  - MH37967/MH/NIMH NIH HHS/United States
GR  - NS12311/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/*analogs & derivatives/pharmacology
MH  - Basal Ganglia/*drug effects/enzymology
MH  - Choline O-Acetyltransferase/metabolism
MH  - Cholinergic Fibers/drug effects
MH  - Female
MH  - Immunoenzyme Techniques
MH  - N-Methylaspartate
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1986/03/26 00:00
MHDA- 1986/03/26 00:01
CRDT- 1986/03/26 00:00
PHST- 1986/03/26 00:00 [pubmed]
PHST- 1986/03/26 00:01 [medline]
PHST- 1986/03/26 00:00 [entrez]
AID - 0006-8993(86)90555-X [pii]
AID - 10.1016/0006-8993(86)90555-x [doi]
PST - ppublish
SO  - Brain Res. 1986 Mar 26;369(1-2):377-82. doi: 10.1016/0006-8993(86)90555-x.